E2B (NC_010956) Virus Tagged ORF Clone

CAT#: VC100431

Myc-DDK-tagged ORF clone of viral ORF for terminal protein precursor pTP [Human adenovirus D], codon optimized for human cell expression, YP_001974468

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100431 represents NCBI reference of YP_001974468 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGAGCGTAAATGATTGCGCCAGACTGACGGGGCAGACAGTCCCCACCATGGACTACTTCCTGC
  CATTGCGAAATATATGGAATAGGGTGAGAGAGTTCCCCAGAGCTTCAACAACAGCCGCAGGAATTACGTG
  GATGAGTCGCTACTTGTACGGTTATCACCGATTGATGTTGGAGGACTTGGCTCCCGGTGCACCAGCTACC
  CAGCGGTGGCCTCTCTATCGGCAGCCCCCACCACACTTTCTGGTCGGTTATCAATATCTTGTTCGCACTT
  GCAACGACTATGTGTTTGATAGTCGCGCCTTCAGCAGGCTCCGATACTCAGAGGTGGTTCAGCCCGGGCT
  CCAGACCGTGAATTGGTCACTCATGGCAAACTGTACATACACCATAAATACAGGTGCATACCATAGGTTT
  GTTGACATGGACGACTTCCAGGACACACTGACCAGAGTACAGCAAGCTATATTGGCCGAGAGAGTTGTCG
  CCGACCTTGCTCTTGTGCAGCCGCTGCGGGGCGTCGGCGTGACCAGGATGGAAGATTCCGCGTCCGCATC
  AGATGATATCGAACGCCTGATGCACGATTACTATAAGAACCTCAGCAGATGCCAAGGGCAAGCATGGGGC
  ATGGCTGAGAGGCTCAGAATTCAACAGGCCGGCCCTAAGGATCTTGTGCTCCTCGCTACTATCCGGCGGC
  TGAAAAACGCATACTTCAACTATATCATAAGCAATAGAAACAGCAACTCTGTACACCGCGCCGCGACTTG
  TTTGTCACTCCCTTGTGACTGCGATTGGCTCGATGCTTTTTTGGAACGATTCTCCGATCCTGTGGATCTG
  GATGCCCTCACCAGCCCAACCCCTCAGCTGATCAGATGTATCGTCTCTGCACTGTCCCTGCCTAACGGGG
  ACCCCCCGCATTACCGGGAAATGACAGGGGGCGTGTTCACTCTCCGCCCTAGAGAAAGAGGCCGGGCTGT
  AACGGAAACTATGCGCCGCAGACGCGGGGAGATGATCGAACGGTTCGTGGATAGACTGCCTGTCAGGCGC
  AGGAGAAGGCGCGCACCCCCTCCACCACCCCCACCTGAGGAGGAAATCGAGGAGGAGGTTGTGATGGAAG
  AGGAAGAGGAGGAGGAGGCACCCGGGGACTTCGAGCGGGAGGTTCGCGCCACCATTGCTGAACTGATTAG
  GCTGTTGGAGGATGAGTTGACCGTTTCTGCAAGAAATGCACAGTTCTTTAACTTTGCCGTTGACTTCTAT
  GAGGCCATGGAAAGACTCGAGGCCATCGGCGACATCTCAGAGATGCCGCTGAGACGGTGGATCATGTATT
  TTTTTGTAACTGAGCACATCGCCACTACCTTGAATTATCTGTTCCAGCGCCTTAGGAATTACGCAGTCTT
  CACCAGACACGTGGAACTGAACCTCGCACAGGTGGTGATGCGAGCCAGGGACGCGGACGGCGATGTCGTT
  TATTCACGAGTTTGGAATGAATCAGGCTTGGGCGCCTTCTCCCAGCTCATGGGGAGAATAAGCAACGATC
  TGGCAGCAACAGTCGAACGGGCTGGTAGAGGAGATCTTCAGGAGGAAGAGATAGAGCAGTTCATGACAGA
  GATCGCGTACCAAGATAACTCAGGAGATGTGCAGGAAATTCTGCGCCAAGCTGCGGTGAACGATGCTGAA
  ATAGACTCAGTGGAGCTCAGTTTCAGGTTTAAGGTTACCGGGCCTGTGGTGTTCACCCAAAGGCGGCAGA
  TACAGGACGTGAATAGGAGGGTGGTGGCTCATGCTAGCGCCCTTCGGGCGCAGCACAGGGATCTCCCAGA
  GAGACATGCAGATGTGCCATTGCCCCCCCTGCCAGCTGGTCCCGAACCTCCCCTGCCACCCGGGGCTCGA
  CCGCGACACCGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100431 representing YP_001974468
  Red=Cloning sites Green=Tags
  
  MALSVNDCARLTGQTVPTMDYFLPLRNIWNRVREFPRASTTAAGITWMSRYLYGYHRLMLEDLAPGAPAT
  QRWPLYRQPPPHFLVGYQYLVRTCNDYVFDSRAFSRLRYSEVVQPGLQTVNWSLMANCTYTINTGAYHRF
  VDMDDFQDTLTRVQQAILAERVVADLALVQPLRGVGVTRMEDSASASDDIERLMHDYYKNLSRCQGQAWG
  MAERLRIQQAGPKDLVLLATIRRLKNAYFNYIISNRNSNSVHRAATCLSLPCDCDWLDAFLERFSDPVDL
  DALTSPTPQLIRCIVSALSLPNGDPPHYREMTGGVFTLRPRERGRAVTETMRRRRGEMIERFVDRLPVRR
  RRRRAPPPPPPPEEEIEEEVVMEEEEEEEAPGDFEREVRATIAELIRLLEDELTVSARNAQFFNFAVDFY
  EAMERLEAIGDISEMPLRRWIMYFFVTEHIATTLNYLFQRLRNYAVFTRHVELNLAQVVMRARDADGDVV
  YSRVWNESGLGAFSQLMGRISNDLAATVERAGRGDLQEEEIEQFMTEIAYQDNSGDVQEILRQAAVNDAE
  IDSVELSFRFKVTGPVVFTQRRQIQDVNRRVVAHASALRAQHRDLPERHADVPLPPLPAGPEPPLPPGAR
  PRHRF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_010956
• ORF Size: 1905 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_010956.1, YP_001974468
• RefSeq ORF: 1905
• Locus ID: 6386271
• MW: 73.0 kDa