early E1A 13s (NC_012959) Virus Tagged ORF Clone

CAT#: VC100146

Myc-DDK-tagged ORF clone of viral ORF for early E1A 13s [Human adenovirus 54], codon optimized for human cell expression, YP_003038597

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: early E1A 13s
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100146 represents NCBI reference of YP_003038597 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCATCTTCATCTGCTGTCCTCAACAGTCCCAATTGACATGGCGGCCCTGCTTCTTGAAGATTACG
  TGAATACCATTCTCGAGGATGAGCTGCATCTGTCCCCCTTCGAGCTCGGCCCGACCCTGCAGGATCTCTA
  CGATTTGGAAGTTAATGCCCAGGAGAACGATCCGAACGAAGAGGCCGTCAACCTCATATTTCCAGAGAGC
  ATGATCCTCCAGGCCGACATCGCCTCAGAGGCAGTGCCTACCTCCGTGTATACGCCCACCCTCCCACCCA
  TCCCAAAGCTGGAGGAGGAAGATAAACTTGATCTGCGGTGCTACGAGGAAGGGTTTCCCCCCAGTGATTC
  CGAAGATGAGAGGGGTGAGCAATCCGTGGCCATTATCTCTGATTATGCATGTGTAGTTGTTGAAAATCAT
  TTCGTGCTTGACAACCCTGAGGTGCCCGGCCAGGGCTGCAAGTCCTGTCAGTACCATAGAGAGCAAACAG
  GCGACCCCAATGCCAGTTGCGCCCTTTGCTATATGAAAATGAGCTTCAGCTTCATCTATTCCCCCGTGTC
  CGAGGATGAATCAAGCCCACTTGAAGAGGATCATCCAAGCCCCCCCGACCTGACAAACGACACTCCCCTC
  CAGGTCAGAAAGCCAACCCCCGTTCGCCCCTCTGGCGAGCGCCGCGCAGCAGTGTACAAGATTGAAGATC
  TGCTGCAGGATGTTGGGGGCAATGAACCCCTGAATCTCTCCCTCAAACGGCCAAGAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100146 representing YP_003038597
  Red=Cloning sites Green=Tags
  
  MRHLHLLSSTVPIDMAALLLEDYVNTILEDELHLSPFELGPTLQDLYDLEVNAQENDPNEEAVNLIFPES
  MILQADIASEAVPTSVYTPTLPPIPKLEEEDKLDLRCYEEGFPPSDSEDERGEQSVAIISDYACVVVENH
  FVLDNPEVPGQGCKSCQYHREQTGDPNASCALCYMKMSFSFIYSPVSEDESSPLEEDHPSPPDLTNDTPL
  QVRKPTPVRPSGERRAAVYKIEDLLQDVGGNEPLNLSLKRPRN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 759 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038597
• RefSeq ORF: 759
• MW: 28.3 kDa