penton (NC_012959) Virus Tagged ORF Clone

CAT#: VC100156

Myc-DDK-tagged ORF clone of viral ORF for penton [Human adenovirus 54], codon optimized for human cell expression, YP_003038607

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: penton
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100156 represents NCBI reference of YP_003038607 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAGAGCTGTGGTGTCATCCCCCCCACCTAGCTACGAGAGCGTGATGGCACAGGCCACTCTGGAGG
  TGCCATTTGTTCCTCCTCGCTATATGGCTCCCACAGAGGGAAGAAATTCAATCAGGTATAGCGAGCTGGC
  ACCGCAGTACGACACCACCCGAGTGTATCTTGTGGACAACAAGAGTGCGGATATAGCCAGTCTGAACTAT
  CAAAATGATCATAGTAACTTTCTCACCACTGTTGTGCAGAATAATGATTTCACCACAGCCGAGGCTTCCA
  CACAGACCATAAACTTCGACGAGAGGTCCCGGTGGGGGGGTGATTTGAAAACCATTCTGCACACTAATAT
  GCCCAACGTCAATGAGTACATGTTTACATCTAAGTTCAAGGCTCGGGTAATGGTGGCACGCAAACACCCC
  GAAGGAGTGGACAACACAGACCTGTCCCAGGATAAGCTCGAGTACCAGTGGTTTGAGTTCACCCTGCCCG
  AAGGAAACTTCAGCGAGACTATGACAATTGACCTGATGAATAATGCTATCCTGGAGAATTACCTGCAGGT
  GGGCCGGCAGAATGGCGTGCTCGAATCAGACATCGGAGTGAAGTTTGACAGCCGCAATTTTAAGTTGGGC
  TGGGACCCTGTTACTAAGCTGGTGATGCCAGGGGTGTATACTTACGAAGCTTTTCACCCCGATGTCGTGT
  TGCTCCCAGGATGCGGGGTCGATTTTACCGAAAGCAGACTCTCTAATCTTTTGGGCATACGCAAGAAGCA
  GCCCTTTCAGGAGGGGTTCCGCATAATGTACGAAGATCTGGAAGGAGGTAATATTCCAGCCCTCCTGAAC
  GTGACTAAATACTTGGAATCCAAAAAGACGCTGCAAAAGGCAGTAGAAAATGCCGCGAAAGTCAATGCCC
  CCGCACGCGGAGACTCATCTGTCCCAAGAGCCGTGGAGAAAGCCGCAGAAAAAGAACTGGAAATCGTACC
  CATCGAGAAAGACGATTCCAACCGAAGTTACAACATTATCCCAGGCACAACAGACACCCTGTACAGGTCC
  TGGTACCTCTCCTATACTTATGGAGACCCCAAGAAAGGTGTGCAGAGTTGGACCTTGTTGACCACTCCGG
  ACGTTACTTGCGGTGCTGAGCAAGTCTACTGGTCCCTTCCTGATCTGATGCAAGACCCCGTGACATTTCG
  GTCTACCCAGCAGGTGTCTAACTACCCGGTTGTCGGGGCTGAACTCATGCCCTTTCGCGCCAAATCCTTT
  TACAACGACCTTGCAGTGTATAGTCAGCTGATCCGCAGTTACACCTCTCTGACACACGTGTTCAACCGAT
  TTCCAGATAACCAGATTCTCTGCCGCCCCCCGGCACCTACAATTACTACCGTTAGCGAAAACGTCCCAGC
  TCTGACCGATCATGGCACTCTGCCATTGAGGTCATCTATTCGCGGGGTCCAGAGGGTAACCGTGACAGAT
  GCCAGGAGGAGAACTTGCCCATACGTTTATAAAGCTCTTGGCATAGTGGCACCCCGCGTCCTCTCCAGCC
  GAACCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100156 representing YP_003038607
  Red=Cloning sites Green=Tags
  
  MRRAVVSSPPPSYESVMAQATLEVPFVPPRYMAPTEGRNSIRYSELAPQYDTTRVYLVDNKSADIASLNY
  QNDHSNFLTTVVQNNDFTTAEASTQTINFDERSRWGGDLKTILHTNMPNVNEYMFTSKFKARVMVARKHP
  EGVDNTDLSQDKLEYQWFEFTLPEGNFSETMTIDLMNNAILENYLQVGRQNGVLESDIGVKFDSRNFKLG
  WDPVTKLVMPGVYTYEAFHPDVVLLPGCGVDFTESRLSNLLGIRKKQPFQEGFRIMYEDLEGGNIPALLN
  VTKYLESKKTLQKAVENAAKVNAPARGDSSVPRAVEKAAEKELEIVPIEKDDSNRSYNIIPGTTDTLYRS
  WYLSYTYGDPKKGVQSWTLLTTPDVTCGAEQVYWSLPDLMQDPVTFRSTQQVSNYPVVGAELMPFRAKSF
  YNDLAVYSQLIRSYTSLTHVFNRFPDNQILCRPPAPTITTVSENVPALTDHGTLPLRSSIRGVQRVTVTD
  ARRRTCPYVYKALGIVAPRVLSSRTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 1548 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038607
• RefSeq ORF: 1548
• MW: 58.3 kDa