pV (NC_012959) Virus Tagged ORF Clone

CAT#: VC100158

Myc-DDK-tagged ORF clone of viral ORF for pV [Human adenovirus 54], codon optimized for human cell expression, YP_003038609

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100158 represents NCBI reference of YP_003038609 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAAACGCAAAATCAAGGAGGAGATGCTTCAGGTGGTAGCTCCAGAAATCTACGGCCCTCCAGCTG
  ACCAGAAGCCTCGGAAGATAAAGAGAGTAAAGAAAAAAGATGAAGTAGGCGAGGGAGCCGTTGAGTTTGT
  ACGGGAGTTTGCTCCACGACGCCGCGTGAATTGGAAAGGCCGACGGGTGCAGCGCGTTCTGAGGCCCGGA
  ACTGCCGTTGTGTTCACACCCGGCGAACGGTCCTCTGTTAGGTCTAAGCGCTCTTACGATGAAGTGTACG
  GCGATAATGATATCCTGGACCAGGCGGCCGAACGCGCGGGAGAATTCGCGTACGGTAAACGCTCTCGGGA
  GGAGGAGCTTATCAGTCTGCCCTTGGATGAATCTAACCCGACTCCGAGTCTCAAGCCAGTGACCCTCCAG
  CAGGTGCTGCCACAGGCCGTGTTGCTGCCATCCCGGGGCGTAAAAAGGGAAGGCGAAAATATGTACCCAA
  CTATGCAAATAATGGTTCCTAAGCGGCGCCGCGTAGAAGATGTCCTCGACACTGTTAAGATGGATGTCGA
  ACCTGAGGTTAAAGTTAGACCGATTAAGCAGGTTGCCCCAGGCCTCGGAGTACAGACCGTGGACATCCAA
  ATACCGACGGATATGGATGTGGATAAAAAACCCAGCACAAGCATCGAGGTCCAGACGGACCCCTGGCTGC
  CCGCCTCCACAGCCTTTACAAGTACTGCCACGGAACCGAGTCGCCGAAGGAGGTGGGGACCGGCCAACCG
  ATTGATGCCCAATTACGTTCTTCACCCAAGCATCATCCCTACCCCGGGGTACCGCGGCACTAGGTACTAT
  GCATCAAGAAGAAGACCGGCAGGAAAACGGCGAAGACGGACCACCACACGCCGGAGGCTGGCCCCCGCCC
  GAGTCCGCCGCGTGACAACACGACAGGGTCGAAGCTTCGTTCTGCCAACAGTGCGGTACCATCCGAGTAT
  TCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100158 representing YP_003038609
  Red=Cloning sites Green=Tags
  
  MSKRKIKEEMLQVVAPEIYGPPADQKPRKIKRVKKKDEVGEGAVEFVREFAPRRRVNWKGRRVQRVLRPG
  TAVVFTPGERSSVRSKRSYDEVYGDNDILDQAAERAGEFAYGKRSREEELISLPLDESNPTPSLKPVTLQ
  QVLPQAVLLPSRGVKREGENMYPTMQIMVPKRRRVEDVLDTVKMDVEPEVKVRPIKQVAPGLGVQTVDIQ
  IPTDMDVDKKPSTSIEVQTDPWLPASTAFTSTATEPSRRRRWGPANRLMPNYVLHPSIIPTPGYRGTRYY
  ASRRRPAGKRRRRTTTRRRLAPARVRRVTTRQGRSFVLPTVRYHPSIL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 984 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038609
• RefSeq ORF: 984
• MW: 37.6 kDa