pVII (NC_012959) Virus Tagged ORF Clone

CAT#: VC100157

Myc-DDK-tagged ORF clone of viral ORF for pVII [Human adenovirus 54], codon optimized for human cell expression, YP_003038608

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100157 represents NCBI reference of YP_003038608 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCATCCTCATCAGCCCTAGTAATAACACTGGCTGGGGTCTTACAAGGCCCAGCACCATGTACGGAG
  GGGCCAAAAAGAGGTCTCAACAACACCCCGTCAGAGTGAGAGGGCACTTCCGCGCACCATGGGGGGCCTA
  TAAAAGGGGAAGAACCTCCGCCCCCGCCGCTGTACGAACGACTGTGGATGATGTGATCGACTCTGTGGTG
  GCTGACGCTCGCAACTACACTCCGGCACCATCCACAGTGGATGCCGTTATTGACAGTGTTGTGGCTGATG
  CCAGAGACTACGCTCGGAGAAAATCAAGAAGAAGGAGAATCGCAAGGAGGCATCGCTCCACACCCGCCAT
  GCGAGCCGCCCGAGCACTGCTGCGAAGAGCTCGACGAACAGGAAGAAGAGCTATGATGCAAGCCGCGAGG
  AGAGCAGCTGCAGCTCCAACACCCACTGGCCGCACTCGACGGCGGGCCGCAGTCGCCGCGGCTGCCGCAA
  TTAGCTCTATGACTCGCCCAAGGCGGGGAAATGTCTACTGGGTCAGGGATTCAATCACAGGAGTCAGGGT
  GCCCGTGCGCACACGACCCCCCCGCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100157 representing YP_003038608
  Red=Cloning sites Green=Tags
  
  MSILISPSNNTGWGLTRPSTMYGGAKKRSQQHPVRVRGHFRAPWGAYKRGRTSAPAAVRTTVDDVIDSVV
  ADARNYTPAPSTVDAVIDSVVADARDYARRKSRRRRIARRHRSTPAMRAARALLRRARRTGRRAMMQAAR
  RAAAAPTPTGRTRRRAAVAAAAAISSMTRPRRGNVYWVRDSITGVRVPVRTRPPRP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 588 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038608
• RefSeq ORF: 588
• MW: 21.7 kDa