29.3 kDa protein (NC_012959) Virus Tagged ORF Clone

CAT#: VC100171

Myc-DDK-tagged ORF clone of viral ORF for 293 kDa protein [Human adenovirus 54], codon optimized for human cell expression, YP_003038622

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 29.3 kDa protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100171 represents NCBI reference of YP_003038622 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGCGTTTACAGCTTGCGTCCTTATCTCCATCGTGACCCTGTCCCTCAGTCAGATTATTAACGTGA
  ATGTCACAAGAGGCGGATCCATCACACTTAACGGCACCTACAAGAATACTACATGGACCCGGTACCACCT
  GGATAGCTGGAAGAATCTGTGCAAGTGGAACATGACGGCCTACAAGTGCTACGACAATGGAAGTATTACG
  ATTACCGCAACCGGAAAAATCACAAGTGGAAAGTACAAGGCAGAATCCTATAAAAACGAGATTAAGAAAA
  GTGTGTTCAAGACAAATAAAACTACCTTCGAAGACTCAGGCAACTACGAACACCAGAAGATCACCTTTTA
  TCAACTGACCATCATCCAGCTTCCGACCACAAAGGTTCCAACAACGACCGCGAGCACTTACACCACCCAG
  CTTAACACCACAGTGCAGAACAGTACTGTCCTGGTGCGGTATCTGCTGAGAGAGGAATCTACCACCCAGC
  AAACAGACGCAACTCTCTCTGCCTTTTCCAGCACTGCCAACCTCACTTCCCTGGCCTGGACCAATGAAAC
  TGGGGTCCTCCTCATGCACGGACAGCCATATAGCGGACTGCACATCCAAATTACCTTTTTGGTGATCTGT
  GGAATTTTTATCCTGGTAGTGCTGCTGTACTTCGTCTGTTGTAAAGCACGAAAGAAATCCCGAAGGCCCA
  TCTATCGGCCAGTGATCGGCAATCCGCAGCCGTTCCAAGTGGAAGGAGGGCTTCGGAACCTGCTGTTTTC
  CTTTTCTGTCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100171 representing YP_003038622
  Red=Cloning sites Green=Tags
  
  MKAFTACVLISIVTLSLSQIINVNVTRGGSITLNGTYKNTTWTRYHLDSWKNLCKWNMTAYKCYDNGSIT
  ITATGKITSGKYKAESYKNEIKKSVFKTNKTTFEDSGNYEHQKITFYQLTIIQLPTTKVPTTTASTYTTQ
  LNTTVQNSTVLVRYLLREESTTQQTDATLSAFSSTANLTSLAWTNETGVLLMHGQPYSGLHIQITFLVIC
  GIFILVVLLYFVCCKARKKSRRPIYRPVIGNPQPFQVEGGLRNLLFSFSVW
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038622
• RefSeq ORF: 783
• MW: 29.4 kDa