8.5 kDa protein (NC_012959) Virus Tagged ORF Clone

CAT#: VC100177

Myc-DDK-tagged ORF clone of viral ORF for 85 kDa protein [Human adenovirus 54], codon optimized for human cell expression, YP_003038628

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 8.5 kDa protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100177 represents NCBI reference of YP_003038628 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTGTTGGTTCTACCCAGCCGCCCGGGTCTCCTCTCCGCTGGGCTATGATCCCGATCACAAGGGCT
  CCAAAAATTTCAAAAAGATGTACGTATCCGTTCCCGTTTCTCGCTACCCAGGTAGCGGTGAAAGCCCTGA
  TGGAAAGAGAGTCCGAGCTCTGGATCCAGCTTTGCCTCTGTTCATCCCCGGTCTGCCTGCAGTGCCCGGT
  GGCAGGCACCCAGCTGGGAGGGTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100177 representing YP_003038628
  Red=Cloning sites Green=Tags
  
  MCCWFYPAARVSSPLGYDPDHKGSKNFKKMYVSVPVSRYPGSGESPDGKRVRALDPALPLFIPGLPAVPG
  GRHPAGRVV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 237 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038628
• RefSeq ORF: 237
• MW: 8.5 kDa