52/55K (NC_012959) Virus Tagged ORF Clone

CAT#: VC100154

Myc-DDK-tagged ORF clone of viral ORF for 52/55K [Human adenovirus 54], codon optimized for human cell expression, YP_003038605

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 52/55K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100154 represents NCBI reference of YP_003038605 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCCCGTCTTGCGGCAGATGAGACCCACTCCCCCAGCCACCACCGCAACTGCTGCCGTGGCAGGAG
  CCGGTGCTGTCCAGCAGCAGACTGAAATGGACCTCGAGGAAGGCGAGGGTTTGGCCAGACTGGGCGCTCC
  TAGCCCCGAGAGACATCCACGGGTGCAGCTGCAGAAGGATGTAAGGCCCGCCTACGTCCCTGCTCAGAAT
  TTGTTCAGAGATCGGTCAGGGGAAGAGCCTGAGGAGATGCGCGATTGCCGATTTCGCGCCGGCCGCGAAC
  TGAGGGAGGGACTGGACCGCCAGAGAGTGCTGCGGGATGATGATTTTGAGCCTAATGAGCAAACTGGGAT
  CTCCCCCGCGAGAGCACACGTTGCCGCCGCGAACCTCGTCACAGCCTATGAACAGACAGTGAAGCAGGAA
  CGGAATTTTCAAAAGAGCTTCAATAACCATGTCAGGACCCTCATCGCCCGAGAAGAAGTGGCTCTCGGTC
  TCATGCATCTCTGGGATCTCGCCGAAGCCATTGTTCAGAATCCGGATAGCAAACCCTTGACTGCGCAACT
  GTTCTTGGTTGTCCAGCATAGTAGGGATAACGAAGCATTTAGGGAAGCACTCCTTAATATCGCAGAGCCC
  GAAGGTCGCTGGCTGCTTGAGCTGATTAACATCTTGCAGAGCATTGTCGTCCAGGAGCGCTCACTCAGCC
  TTGCTGAGAAAGTTGCTGCCATAAATTATAGTGTTCTGTCTCTGGGCAAATTTTATGCTCGCAAGATCTA
  TAAGACCCCCTATGTTCCCATCGATAAAGAAGTCAAAATTGACAGCTTCTACATGAGAATGGCGCTCAAG
  GTCCTTACTCTGAGTGATGACCTGGGCGTATACAGAAATGACAGGATACATAAGGCTGTTTCAACTTCCA
  GACGCAGGGAGCTGAGCGACCGGGAGCTTATGCTGAGTCTGCGAAGAGCCCTCGTAGGGGGTGCCGCGTC
  TGGCGAGGAGTCACACTTCGACATGGGCGCAGATCTCCACTGGCAGCCTAGCAGGAGAGCTTTGGAAGCT
  GCGTATGGCCCTGAGGACCTGGATGAGGATGAGGAGGAGGAAGAAGATGCTCCTGCTGCTGGGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100154 representing YP_003038605
  Red=Cloning sites Green=Tags
  
  MHPVLRQMRPTPPATTATAAVAGAGAVQQQTEMDLEEGEGLARLGAPSPERHPRVQLQKDVRPAYVPAQN
  LFRDRSGEEPEEMRDCRFRAGRELREGLDRQRVLRDDDFEPNEQTGISPARAHVAAANLVTAYEQTVKQE
  RNFQKSFNNHVRTLIAREEVALGLMHLWDLAEAIVQNPDSKPLTAQLFLVVQHSRDNEAFREALLNIAEP
  EGRWLLELINILQSIVVQERSLSLAEKVAAINYSVLSLGKFYARKIYKTPYVPIDKEVKIDSFYMRMALK
  VLTLSDDLGVYRNDRIHKAVSTSRRRELSDRELMLSLRRALVGGAASGEESHFDMGADLHWQPSRRALEA
  AYGPEDLDEDEEEEEDAPAAGY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038605
• RefSeq ORF: 1116
• MW: 42.0 kDa