polymerase (NC_012959) Virus Tagged ORF Clone

CAT#: VC100152

Myc-DDK-tagged ORF clone of viral ORF for polymerase [Human adenovirus 54], codon optimized for human cell expression, YP_003038603

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: polymerase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100152 represents NCBI reference of YP_003038603 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAATGTGTCGATACAGCTACTAACTCACCCGTGGAAATCAAGTATCATCTGCATCTGAAGCACGCCC
  TGTCACGGCTGTATGAAGTGAACCTGAAGACTCTGCCCCCTGATCTGGATCTCCGAAACACCATGGACTC
  CTCCCAACTGCGCGCACTGGTTTTTGCACTTCGCCCTAGACGCGCAGAAATCTGGACGTGGCTTCCAAGG
  GGGTTGGTGTCACTGTCCGTGTTGGAGGAGCCCCAGGGGAAGTCTTACGCTGGAGAGCACGAAAACCATC
  AGCCTGGCCCCCCTCTCCTTAAATTTCTGCTGAAGGGGCGGGCAGTGTACTTGGTGGACGAGGTCCAGCC
  CGTGCAGCGGTGCGAATATTGCGGACGCTTCTATAAGCATCAGCACGAGTGTAGCGTTAGGCGCAGAGAT
  TTTTACTTTCATCACATAAATTCTCATAGCAGCAACTGGTGGCAAGAGATTCAGTTCTTCCCAATCGGAA
  GCCATCCCCGGACTGAGCGATTGTTTGTGACCTATGATGTGGAGACCTACACATGGATGGGCTCCTTTGG
  CAAGCAGCTGGTCCCCTTCATGCTGGTGATGAAGTTTTCCGGGGACCCAAAGCTGGTCGCTCTTGCTCGG
  GACCTGGCCGTGCGACTGCGGTGGGATCGCTGGGAGAGAGATCCTCTGACATTTTATTGTATCACGCCCG
  AAAAAATGGCAGTGGGACAGCAATTCAGACTCTTCAGAGACGAACTGCAAACTCTTATGGCAAGGGAGTT
  GTGGGTTTCCTTTATGCAAGCTAACCCACATCTGCAGGAGTGGGCGCTGGAGCAACATGGTCTGCAGTGT
  CCTGAGGATTTGACTTACGAAGAGTTGAAGAAACTCCCCCACATAAAGGGACGACCTAGATTTATGGAAC
  TCTACATAGTCGGCCACAACATCAACGGTTTTGATGAAATTGTGCTGGCCGCCCAGGTCATCAACAACAG
  AGCCTGCGTCCCAGGTCCATTCCGGATTACTAGGAATTTTATGCCCCGCGCAGGAAAAATCCTGTTCAAC
  GATGTCACCTTCGCCCTGCCTAATCCCTTGAGTAAAAAGAGAACCGACTTCGAGCTCTGGGAGCACGGTG
  GCTGTGATGACTCAGACTTCAAGTACCAGTTTCTCAAGGTAATGGTCAGAGACACATTCGCCCTCACCCA
  CACTTCCCTGCGGAAGGCTGCCCAGGCCTATGCGCTGCCAGTGGAGAAAGGGTGCTGTCCATATAAGGCC
  GTTAACCACTTTTACATGCTCGGAAGTTACCGGGCAGACGACCGAGGCTTCCCTTTGAGAGAGTACTGGA
  AAGATGATGAAGAGTATGCCTTGAACCGCGAACTGTGGAAGAAAAAAGGAGAAGCAGGGTACGACATCAT
  CCGGGAAACACTGGACTACTGTGCTATGGATGTGCTCGTCACTGCAGAACTCGTCGCTAAGTTGCAGGAT
  AGTTATGCCCACTTTATCAGGGATAGCGTGAGACTTCCACATGCTCATTTCAACATATTCCAGAGGCCCA
  CCATCTCAAGTAATTCTCATGCCATTTTCCGACAGATTGTCTTCCGCGCTGAGCAGCCACAGCGCACGAA
  TCTTGGGCCTGCGTTCCTGGCACCCTCTCACGAGCTGTATGACTACGTGCGCGCCTCTATTCGGGGTGGA
  AGGTGTTACCCAACTTATATTGGCATACTGTCAGAACCAATCTATGTTTACGACATTTGTGGGATGTACG
  CCTCCGCACTCACTCATCCGATGCCTTGGGGGCCTCCACTGAACCCATGCGAACGGGCTCTTGCTGCCAG
  GAACTGGCAAATGGCACTGGATGATGCTTCCCTCAAGATTGATTACTTCGATAAGGAATTGTGTCCCGGG
  ATTTTCACAATCGACGCCGATCCACCCGACGAACATCTGCTGGATGTGCTTCCCCCGTTTTGCTCACGCA
  AAGGGGGCAGGCTCTGCTGGACAAACGAACCTCTGCGCGGAGAGGTGGCCACATCAGTTGATCTTGTCAC
  ACTCCACAACCGGGGCTGGAGGGTAAGGATCGTACCTGATGAAAGGACGACAGTGTTTCCGGAATGGAAG
  TGTGTCGCTAGAGAATACGTCCAGCTGAATATCGCTGCCAAAGAACGCGCTGACCGAGATAAAAATCAAA
  CCATGAGATCTATTGCCAAACTGCTGAGTAACGCTCTCTACGGCAGCTTCGCTACCAAGTTGGATAACAA
  AAAGATCGTTTTTAGCGATCAGATGGATGAGAGCCTTCTTAAGAGCATTGCAGCTGGCCAGGCTAACATT
  AAGTCCTCAAGCTTTCTGGAGACGGACAATCTGAGCGCCGAGGTGATGCCGGCCCTCGAACGGGAGTACT
  TGCCCCAGCAACTGGCCTTGGTTGACTCCGATGCTGAAGAGAGCGAAGACGAACACCGCCCGGCACCCTT
  CTATACTCCACCTTCCGGAACCCCCGGGCACGTGGCCTATACATACAAGCCGATTACTTTCCTGGATGCG
  GAAGAAGGCGATATGTGTCTGCACACCGTCGAGAAGGTGGATCCCCTGGTTGAGAATGACAGGTACCCCA
  GCCACGTCGCTAGTTTTGTCCTGGCCTGGACAAGAGCCTTTGTTAGCGAGTGGTCTGAATTTCTTTACGA
  GGAAGACCGAGGAACTCCCCTGCAGGACAGACCCATAAAGTCCGTTTACGGGGATACGGATTCACTGTTC
  GTTACAGAACGAGGCCACAGGCTGATGGAAACTAGGGGTAAGAAAAGGATTAAAAAGAACGGAGGTAAAC
  TGGTCTTTGATCCTAAGCAGCCCGAACTGACCTGGCTGGTGGAGTGCGAAACAGTTTGCGCGCATTGCGG
  CGCAGACGCATTTGCGCCCGAAAGCGTTTTTCTCGCTCCCAAGCTCTACGCTCTGCAGAGCCTGCTTTGC
  CCAGCTTGCGGCAGGTTCAGCAAGGGCAAGCTGCGCGCCAAAGGACACGCCGCAAAGGCCCTGAATTACC
  AGTTGATGGTCAACTGCTATCTTGCAGACGCCCAGGGCGAAGACAGAGCTAGGTTTAGTACTTCTCGCAT
  GAGCCTGAAGCGGACCCTTGCATCCGCTCAGCCAGGTGCACACCCCTTCACTGTGACGGAGACGACCCTG
  ACACGGACTCTGCGCCCGTGGAAAGACATGACCCTGGCTGCCCTGGATGCACACAGGCTGGTTCCTTATT
  CCAGAAGCAGACCTAACCCTCGCAACGAGGAAGTGTGTTGGATCCAGATGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100152 representing YP_003038603
  Red=Cloning sites Green=Tags
  
  MQCVDTATNSPVEIKYHLHLKHALSRLYEVNLKTLPPDLDLRNTMDSSQLRALVFALRPRRAEIWTWLPR
  GLVSLSVLEEPQGKSYAGEHENHQPGPPLLKFLLKGRAVYLVDEVQPVQRCEYCGRFYKHQHECSVRRRD
  FYFHHINSHSSNWWQEIQFFPIGSHPRTERLFVTYDVETYTWMGSFGKQLVPFMLVMKFSGDPKLVALAR
  DLAVRLRWDRWERDPLTFYCITPEKMAVGQQFRLFRDELQTLMARELWVSFMQANPHLQEWALEQHGLQC
  PEDLTYEELKKLPHIKGRPRFMELYIVGHNINGFDEIVLAAQVINNRACVPGPFRITRNFMPRAGKILFN
  DVTFALPNPLSKKRTDFELWEHGGCDDSDFKYQFLKVMVRDTFALTHTSLRKAAQAYALPVEKGCCPYKA
  VNHFYMLGSYRADDRGFPLREYWKDDEEYALNRELWKKKGEAGYDIIRETLDYCAMDVLVTAELVAKLQD
  SYAHFIRDSVRLPHAHFNIFQRPTISSNSHAIFRQIVFRAEQPQRTNLGPAFLAPSHELYDYVRASIRGG
  RCYPTYIGILSEPIYVYDICGMYASALTHPMPWGPPLNPCERALAARNWQMALDDASLKIDYFDKELCPG
  IFTIDADPPDEHLLDVLPPFCSRKGGRLCWTNEPLRGEVATSVDLVTLHNRGWRVRIVPDERTTVFPEWK
  CVAREYVQLNIAAKERADRDKNQTMRSIAKLLSNALYGSFATKLDNKKIVFSDQMDESLLKSIAAGQANI
  KSSSFLETDNLSAEVMPALEREYLPQQLALVDSDAEESEDEHRPAPFYTPPSGTPGHVAYTYKPITFLDA
  EEGDMCLHTVEKVDPLVENDRYPSHVASFVLAWTRAFVSEWSEFLYEEDRGTPLQDRPIKSVYGDTDSLF
  VTERGHRLMETRGKKRIKKNGGKLVFDPKQPELTWLVECETVCAHCGADAFAPESVFLAPKLYALQSLLC
  PACGRFSKGKLRAKGHAAKALNYQLMVNCYLADAQGEDRARFSTSRMSLKRTLASAQPGAHPFTVTETTL
  TRTLRPWKDMTLAALDAHRLVPYSRSRPNPRNEEVCWIQMP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 3273 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038603
• RefSeq ORF: 3273
• MW: 125.4 kDa