12.2 kDa protein (NC_012959) Virus Tagged ORF Clone

CAT#: VC100167

Myc-DDK-tagged ORF clone of viral ORF for 122 kDa protein [Human adenovirus 54], codon optimized for human cell expression, YP_003038618

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 12.2 kDa protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100167 represents NCBI reference of YP_003038618 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTATGGGGATTCCGCTGAGTTGGCAAGATTGCGGCACCTCGACCACTGTAGGAGATTGAGATGTT
  TCGCACGAGAAAGTTGTGGCCTGGTGTACTTCAAGCTGCCTGAAGAACACCCTAACGGCCCCGCTCACGG
  CGTGAGGATAACAGTCGAAGGCACCACCAAGAGCTATCTTGTCCGGTTTTTCACACAGCAACCCTTCCTC
  GTTGAACGAGACCGAGGGGCCACTACTTACACAGTATACTGCATCTGCCCTACGCCAAAACTGCACGAGA
  ACTTTTGTTGTACATTGTGCGGAGAATTCAACAAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100167 representing YP_003038618
  Red=Cloning sites Green=Tags
  
  MSYGDSAELARLRHLDHCRRLRCFARESCGLVYFKLPEEHPNGPAHGVRITVEGTTKSYLVRFFTQQPFL
  VERDRGATTYTVYCICPTPKLHENFCCTLCGEFNKS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 318 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038618
• RefSeq ORF: 318
• MW: 12.2 kDa