Early E1A 12s (NC_012959) Virus Tagged ORF Clone

CAT#: VC100147

Myc-DDK-tagged ORF clone of viral ORF for Early E1A 12s [Human adenovirus 54], codon optimized for human cell expression, YP_003038598

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Early E1A 12s
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100147 represents NCBI reference of YP_003038598 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCACCTCCACCTGCTGAGCTCTACTGTGCCTATCGATATGGCAGCCCTCCTCTTGGAAGATTATG
  TGAACACTATACTCGAGGACGAACTCCATCTGTCTCCTTTTGAGCTCGGCCCAACTCTGCAGGACCTGTA
  TGATCTGGAAGTCAATGCCCAGGAAAACGACCCCAATGAGGAGGCCGTTAACTTGATCTTCCCTGAGTCC
  ATGATCCTGCAGGCCGACATTGCAAGCGAAGCCGTTCCTACATCTGTCTATACTCCCACTTTGCCACCCA
  TTCCCAAGCTGGAGGAAGAGGATAAGCTTGATTTGAGGTGCTATGAGGAGGGATTTCCACCGAGCGACAG
  TGAAGATGAGAGAGGACCTGTGTCTGAAGACGAAAGTAGTCCCCTGGAGGAGGACCATCCCTCCCCTCCT
  GATCTGACCAATGACACCCCCCTGCAAGTACGGAAACCCACTCCTGTTAGACCAAGCGGAGAGCGACGCG
  CCGCAGTGTACAAAATTGAGGATCTGCTGCAGGACGTGGGGGGAAACGAGCCCTTGAACCTGTCTCTTAA
  GAGACCCCGGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100147 representing YP_003038598
  Red=Cloning sites Green=Tags
  
  MRHLHLLSSTVPIDMAALLLEDYVNTILEDELHLSPFELGPTLQDLYDLEVNAQENDPNEEAVNLIFPES
  MILQADIASEAVPTSVYTPTLPPIPKLEEEDKLDLRCYEEGFPPSDSEDERGPVSEDESSPLEEDHPSPP
  DLTNDTPLQVRKPTPVRPSGERRAAVYKIEDLLQDVGGNEPLNLSLKRPRN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 573 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038598
• RefSeq ORF: 573
• MW: 21.4 kDa