pIIIa (NC_012959) Virus Tagged ORF Clone

CAT#: VC100155

Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus 54], codon optimized for human cell expression, YP_003038606

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pIIIa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100155 represents NCBI reference of YP_003038606 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCAGCAGCAGGCTCCTGATCCTGCAATACGCGCCGCGCTCCAGTCTCAGCCTTCCGGACTCGCGT
  CCGACGACTGGGAAGCCGCCATGCAAAGGATTATGGCTCTGACAACACGGAATCCCGAGTCTTTTCGCCA
  GCAGCCCCAGGCTAATAGGCTGTCCGCAATTCTGGAGGCTGTAGTGCCAAGCAGGACAAACCCCACTCAC
  GAGAAAGTGCTCGCAATTGTCAACGCTCTTGCTGAAAATAAAGCCATAAGGCCCGACGAGGCCGGACTCG
  TGTATAACGCCCTCCTGGAGAGGGTCGGAAGGTACAACAGCACAAATGTTCAGAGCAATCTGGACCGACT
  TGTGACCGACGTGCGAGAGGCGGTCGCCCAGCGGGAGCGGTTCAAAAATGAGGGCCTCGGGAGCTTGGTC
  GCTCTTAATGCCTTTTTGGCTACCCAACCAGCCAACGTCCCACGGGGTCAGGATGACTACACTAACTTTA
  TAAGTGCACTCAGGCTGATGGTGACAGAGGTCCCTCAATCTGAAGTATATCAGTCTGGCCCAGATTACTT
  TTTCCAGACTTCCCGGCAGGGCCTGCAGACTGTCAACCTGTCCCAGGCCTTTAAAAACCTTCGAGGGCTG
  TGGGGCGTACAGGCACCCGTGGGTGACAGGAGCACAGTTTCAAGTCTTCTTACCCCTAACAGCCGCCTTC
  TGTTGTTGCTGATTGCACCATTTACGGACTCAGGAAGCGTGAATAGAAATAGTTACCTGGGCCACCTGCT
  TACCCTGTATAGGGAGGCGATCGGCCAGGCCCAGGTGGATGAACAGACATTTCAGGAGATAACCTCCGTT
  AGTAGAGCCCTGGGGCAGAATGACACAGACAGTCTGCGAGCCACTCTTAACTTTCTGCTGACCAATAGGC
  AACAAAAGATCCCTCCACAGTACGCTCTCAGCGCAGAAGAGGAACGCATCCTTCGCTACGTCCAGCAATC
  CGTGGGCCTGTTCCTTATGCAGGAAGGGGCCACCCCCTCTGCAGCATTGGACATGACTGCTCGGAATATG
  GAACCCTCCATGTACGCTGCTAATCGCCCCTTTATCAACAAACTGATGGACTACCTGCATCGCGCCGCTA
  GTATGAACAGTGATTATTTTACCAACGCAATACTTAACCCTCACTGGCTCCCTCCGCCGGGATTCTACAC
  CGGGGAGTACGACATGCCGGACCCAAACGACGGGTTCCTGTGGGATGATGTGGACTCCGCCGTGTTCCTC
  CCCGCATTCCAGAAAAGACAGGAAGCCATGAGGACACCATCAGAAGGCGCCGTGGGCCGGAGTCCTTTTC
  CCTCTCTGGGGTCTCTGCACAGCCTGCCCAACTCTGTCAATAGTGGGAGGGTTTCCAGGCCACATCTCCT
  GGGTGAGGATGAATATCTTAACGATTCATTGCTGCGGCCGCCCAGGGCCAAGAACGCTATGGCCAACAAC
  GGCATCGAATCTCTCGTTGATAAGCTCAATCGCTGGAAGACTTATGCACAAGATCACCGGGACGCCCCCG
  CTCCCAGAAGGCAAAGACACGACAGACAGCGGGGTCTGGTCTGGGATGACGAAGACAGTGCAGACGACAG
  TTCCGTCCTTGACCTCGGCGGCAGCGGAGGAGGCGCCAATCCCTTTGCTCACCTGCAGCCTAGATTGGGG
  CAGCGCATGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100155 representing YP_003038606
  Red=Cloning sites Green=Tags
  
  MSQQQAPDPAIRAALQSQPSGLASDDWEAAMQRIMALTTRNPESFRQQPQANRLSAILEAVVPSRTNPTH
  EKVLAIVNALAENKAIRPDEAGLVYNALLERVGRYNSTNVQSNLDRLVTDVREAVAQRERFKNEGLGSLV
  ALNAFLATQPANVPRGQDDYTNFISALRLMVTEVPQSEVYQSGPDYFFQTSRQGLQTVNLSQAFKNLRGL
  WGVQAPVGDRSTVSSLLTPNSRLLLLLIAPFTDSGSVNRNSYLGHLLTLYREAIGQAQVDEQTFQEITSV
  SRALGQNDTDSLRATLNFLLTNRQQKIPPQYALSAEEERILRYVQQSVGLFLMQEGATPSAALDMTARNM
  EPSMYAANRPFINKLMDYLHRAASMNSDYFTNAILNPHWLPPPGFYTGEYDMPDPNDGFLWDDVDSAVFL
  PAFQKRQEAMRTPSEGAVGRSPFPSLGSLHSLPNSVNSGRVSRPHLLGEDEYLNDSLLRPPRAKNAMANN
  GIESLVDKLNRWKTYAQDHRDAPAPRRQRHDRQRGLVWDDEDSADDSSVLDLGGSGGGANPFAHLQPRLG
  QRMF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 1692 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038606
• RefSeq ORF: 1692
• MW: 62.7 kDa