17.6 kDa protein (NC_012959) Virus Tagged ORF Clone

CAT#: VC100169

Myc-DDK-tagged ORF clone of viral ORF for 176 kDa protein [Human adenovirus 54], codon optimized for human cell expression, YP_003038620

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 17.6 kDa protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100169 represents NCBI reference of YP_003038620 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGCCTCCTTCTCATCATTCTGAGTCTCGTTGGTGGTGTCCTGTCTTGCCATGAGCAACCAAGGT
  GCAATATTACGACGGGGAACGAGCGGTCTGTGATCTGTACAGTAGTGATCAAGTGTGAACACCAGTGTCC
  TCTCAATATTACCTTTAAAAATAGGACAATGGGCAACGCCTGGGTAGGCTACTGGGAACCCGGGGATGAA
  CAGAACTATACAGTGACAGTACACGGGTCTGATGGGAACCACACTTTCGGGTTTAAATTTATCTTCGAGG
  TAATGTGTGACATAACACTTCATGTTGCTCGCCTGCACGGTCTGTGGCCTCCTACAAAAGAGAACATGGT
  TGGCTTTAGTCTGGCGTTTGTAATCATGGCGTGCTTCATGTCCGGTCTGCTGGTGGGAGCGCTCATGTGG
  TTCCTCAAATGTAAACCACGATACGGCAATGAACAGAAAGAAAAGCTGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100169 representing YP_003038620
  Red=Cloning sites Green=Tags
  
  MKGLLLIILSLVGGVLSCHEQPRCNITTGNERSVICTVVIKCEHQCPLNITFKNRTMGNAWVGYWEPGDE
  QNYTVTVHGSDGNHTFGFKFIFEVMCDITLHVARLHGLWPPTKENMVGFSLAFVIMACFMSGLLVGALMW
  FLKCKPRYGNEQKEKLL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 471 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038620
• RefSeq ORF: 471
• MW: 17.7 kDa