pTP (NC_012959) Virus Tagged ORF Clone

CAT#: VC100153

Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus 54], codon optimized for human cell expression, YP_003038604

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pTP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100153 represents NCBI reference of YP_003038604 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTGAGCGTGAATGACTGCGCGCGCCTTACAGGACAAACTGTTCCTACCATGGATTATTTCTTGC
  CTCTGCGAAACATCTGGAACAGGGTTCGGGAGTTCCCCCGGGCCAGCACTACCGCCGCCGGTATAACATG
  GATGTCTCGCTACTTGTATGGGTATCACCGGCTCATGTTGGAAGACCTGGCCCCTGGTGCCCCCGCAACT
  CAACGCTGGCCCCTCTATAGACAACCGCCCCCCCATTTTCTTGTGGGATATCAGTACTTGGTACGAACGT
  GCAATGATTACGTGTTTGATAGCCGAGCCTTTAGCCGGTTGCGGTATAGCGAGGTTGTCCAGCCAGGGCT
  GCAGACTGTCAATTGGTCCCTTATGGCAAACTGTACCTATACTATCAATACCGGAGCCTACCACAGGTTC
  GTCGATATGGACGATTTTCAGGATACGCTCACAAGAGTGCAGCAGGCAATTTTGGCGGAGCGAGTGGTAG
  CCGACTTGGCTCTGGTGCAGCCGCTGAGGGGCGTGGGAGTTACACGCATGGAAGATAGCGCCTCAGCCGC
  AAATGACGACATAGAGAGACTGATGCATGATTATTACAAGAATTTGTCCAGATGCCAGGGACAGGCATGG
  GGCATGGCCGAACGCCTGAGAATCCAGCAAGCCGGACCAAAAGACCTCGTGCTGCTCGCCACCATCAGGC
  GCCTCAAGAATGCTTACTTCAACTACATTATCTCCAACCGCAATTCCAACTCCGTGCATCGCGCCGCCAC
  ATGCCTTAGTCTGCCGTGCGATTGCGACTGGCTGGACGCATTTCTCCAGAGGTTTTCCGACCCTGTGGAT
  CTGGATGCGCTCACCTCCCCTACTCCACAGCTGATCCGATGTATCGTGAGTGCGTTGTCTCTGCCGAACG
  GAGACCCCCCCTATTATCGCGAAATGACCGGTGGGGTCTTTACACTGCGACCTAGAGAACAGGGACGCGC
  AGTGACCGAGACCATGCGAAGACGCCGAGGGGAAATGATTGAGCGGTTCGTCGATAGACTTCCTGTGAGG
  CGCCGGCGCAGACGCGCACCGCCCCCACCCCCTCCCCCCGAAGAGATTGAGGAAGAAGTCGTGATGGAGG
  AAGAGCAGGAGGAGGCACCAGGGGACTTCGAAAGGGAGGTGCGAGCCACGATAGCAGAGCTTATACGCCT
  GTTGGAGGATGAGTTGACCGTGTCAGCCAGAAACGCACAGTTTTTTAATTTCGCCGTTGATTTCTACGAA
  GCTATGGAACGACTCGAAGCTATTGGCGACATATCCGAGATGCCCCTTAGACGGTGGATCATGTATTTCT
  TTGTGACGGAGCACATCGCCACTACTCTGAACTATCTTTTTCAACGGCTTAGGAATTACGCCGTTTTCAC
  TAGACACGTGGAGCTGAACCTTGCGCAAGTGGTTATGAGAGCACGAGACGTGGATGGGGATGTGGTGTAT
  AGCAGAGTGTGGAACGAGTCCGGACTGGGGGCTTTCTCACAACTTATGGGACGCATTTCTAACGACCTTG
  CTGCCACGGTTGAACGGGCCGGCAGAGGCGATCTCCAGGAAGAGGAAATCGAGCAATTCATGTCAGAGAT
  CGCCTATCAAGACAATAGCGGCGACGTTCAGGAGATCCTTAGGCAGGCAGCTGTAAATGACGCAGAGATT
  GACTCAGTCGAGCTCAGCTTTAGGTTTAAAGTGACAGGACCTGTCGTGTTCACACAGAGGCGCCAGATAC
  AAGACGTCAACCGCAGGGTTGTTGCTCATGCAAGCGCTCTTCGAGCACAACATAGAGATCTGCCTGAAAG
  ACACGCGGACGTTCCACTCCCCCCCCTGCCTACTGGGCCTGAACCTCCACTTCCTCCTGGCGCGCGGCCT
  CGCCACCGGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100153 representing YP_003038604
  Red=Cloning sites Green=Tags
  
  MALSVNDCARLTGQTVPTMDYFLPLRNIWNRVREFPRASTTAAGITWMSRYLYGYHRLMLEDLAPGAPAT
  QRWPLYRQPPPHFLVGYQYLVRTCNDYVFDSRAFSRLRYSEVVQPGLQTVNWSLMANCTYTINTGAYHRF
  VDMDDFQDTLTRVQQAILAERVVADLALVQPLRGVGVTRMEDSASAANDDIERLMHDYYKNLSRCQGQAW
  GMAERLRIQQAGPKDLVLLATIRRLKNAYFNYIISNRNSNSVHRAATCLSLPCDCDWLDAFLQRFSDPVD
  LDALTSPTPQLIRCIVSALSLPNGDPPYYREMTGGVFTLRPREQGRAVTETMRRRRGEMIERFVDRLPVR
  RRRRRAPPPPPPPEEIEEEVVMEEEQEEAPGDFEREVRATIAELIRLLEDELTVSARNAQFFNFAVDFYE
  AMERLEAIGDISEMPLRRWIMYFFVTEHIATTLNYLFQRLRNYAVFTRHVELNLAQVVMRARDVDGDVVY
  SRVWNESGLGAFSQLMGRISNDLAATVERAGRGDLQEEEIEQFMSEIAYQDNSGDVQEILRQAAVNDAEI
  DSVELSFRFKVTGPVVFTQRRQIQDVNRRVVAHASALRAQHRDLPERHADVPLPPLPTGPEPPLPPGARP
  RHRF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 1902 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038604
• RefSeq ORF: 1902
• MW: 72.9 kDa