14.1 kDa protein (NC_012959) Virus Tagged ORF Clone

CAT#: VC100178

Myc-DDK-tagged ORF clone of viral ORF for 141 kDa protein [Human adenovirus 54], codon optimized for human cell expression, YP_003038629

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 14.1 kDa protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100178 represents NCBI reference of YP_003038629 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCTGCCCATCCTGCCCCCACCTCCACTTAACGATAGGCAGGGCTCCATCAATTGGATGGGAATGG
  CCTACAGGGTCCTCGCCGACGTTCTTAGAGGAATCCGGATGGACGGTCTGTTTGTGAGCTCCGACACCGA
  GGAACTCCTGCACAATCTGTGGGAGTGGATGTACTTTAGTTGGATGGTGGAAAGGCAACAGAGGAAGGAT
  GGCCGCCGGAGGGGCATTTGCTGCTCACGAGCTACCTTCTGTTGGCAGAAATATAATGAGGTTAGAAAGC
  GCGTGCACTATAATGAACATCGGGGAACAATTGACCTCGCTCCCCCTAGTTCCATCAGCCAAGGTCCTTT
  TATTACCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100178 representing YP_003038629
  Red=Cloning sites Green=Tags
  
  MVLPILPPPPLNDRQGSINWMGMAYRVLADVLRGIRMDGLFVSSDTEELLHNLWEWMYFSWMVERQQRKD
  GRRRGICCSRATFCWQKYNEVRKRVHYNEHRGTIDLAPPSSISQGPFITI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 360 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038629
• RefSeq ORF: 360
• MW: 14.1 kDa