34 kDa protein (NC_012959) Virus Tagged ORF Clone

CAT#: VC100176

Myc-DDK-tagged ORF clone of viral ORF for 34 kDa protein [Human adenovirus 54], codon optimized for human cell expression, YP_003038627

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 34 kDa protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100176 represents NCBI reference of YP_003038627 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTACAGAAGAACAAAGCACGTTGCTCCGCCACCACCCTTATCGGCGCGCCCGGCTGTCCCGCTATG
  ATAAAGAAACAAGGGCCTCCCTGACGGAACAGCATCCCTTGCTGCCCGATTGTGACCACGCAGATTACCA
  CAATGTGTCCAGTGTCAGAGGACTTCCATGCGCGGCAGGGTTCACTCTGCTCCAAGAGTTTCCGGTGCCT
  TGGGACATGATACTGACTACAAAAGAGGTAAAAATTCTGAAGCGATGTATGTCTGTTTGTCTTTGCCCCG
  CCACTCTCGATCTTGTCCGAGCGCAGATGGTGTCTGGTTATGAGAGATGGATCCTGCACTGTCACTGTTC
  CTCCCCAGGTTCCTTGCAGTGTCGGGCCGGAGGAACACTGCTGGCTGTCTGGTTCCGCCGGGTCATATAC
  GGTTGCATGTTCAACCAAAGATTCCCATGGTACAGACAGATAGTCAACCGGAACATGCCGAAGGAGATAA
  TGTACATGGGCAGCGTGTTTATCCGGGGGAGACACCTCATTTACTGTAGGATATGGTACGACGGTCACGT
  GGGGTCAATCATCCCCAACATATCCTTTGGTTGGAGCACCCTCAACTACGGTTTGCTTAACAACATCATG
  ATTATGTGCTGTACGTATTGCGAAAATATGTCAGAAATCAGGATGAGGTGCTGTGCCCGGAGGACTAGAC
  GACTTATGCTCCGAGCCGTGGGAATAATTGTGAAGGAAACCTGCGACCCTGATCCAATATGTTCCAGCAG
  GACTGAGCCACGGCGCCAGCGGCTGCTGAGGGCTCTGATGGAACGGCATAGACCTATCCTCTTTAGCGAA
  TACGAATCCGTGCGGTCCAGTCACTCTACACGACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100176 representing YP_003038627
  Red=Cloning sites Green=Tags
  
  MSTEEQSTLLRHHPYRRARLSRYDKETRASLTEQHPLLPDCDHADYHNVSSVRGLPCAAGFTLLQEFPVP
  WDMILTTKEVKILKRCMSVCLCPATLDLVRAQMVSGYERWILHCHCSSPGSLQCRAGGTLLAVWFRRVIY
  GCMFNQRFPWYRQIVNRNMPKEIMYMGSVFIRGRHLIYCRIWYDGHVGSIIPNISFGWSTLNYGLLNNIM
  IMCCTYCENMSEIRMRCCARRTRRLMLRAVGIIVKETCDPDPICSSRTEPRRQRLLRALMERHRPILFSE
  YESVRSSHSTRL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 876 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038627
• RefSeq ORF: 876
• MW: 34.2 kDa