80.2 kDa protein (NC_012959) Virus Tagged ORF Clone

CAT#: VC100164

Myc-DDK-tagged ORF clone of viral ORF for 802 kDa protein [Human adenovirus 54], codon optimized for human cell expression, YP_003038615

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 80.2 kDa protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100164 represents NCBI reference of YP_003038615 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGCAGCCTAGAAAGCAAGAGCAGGAAGAGGAGAACCTCACCACTCATGAACAGCCCAAAATTA
  AACAGGATCTGGGGTTGGAAGAGCCTGCCAGGCTGGAGCCACCACAAGACGAGCAGAAGGAAACTAACGC
  CGGACTCGAGCACGGCTACTTGGGGGGACAGGAGGACGTTCTGTTGAAGCATCTGCAGAGGCAATCTTTT
  ATTCTGCGCGACGCCCTGGCCGACCGCTCAAAAACTCCACTTAGCGTGCAGGAGCTGTGTCGAGCCTACG
  AACTGAACCTCTTTTCCCCCAGAGTGCCACCCAAGAGGCAGCCGAACGGGACATGCGAACCAAATCCTAG
  ACTCAACTTCTATCCAGTGTTTGCCGTGCCAGAGGCCTTGGCCACATACCATATCTTCTTCAAGAACCAA
  AAAATTCCTGTGTCATGTAGGGCTAATAGAACGAGGGCAGACGCATTGTTGGCCCTCGGACCTGGTGCCC
  GGATTCCTGATATTGCCTCACTCGAAGAGGTCCCAAAAATCTTTGAAGGACTGGGCCGAGACGAAACAAG
  AGCCGCTAATGCTCTGAAGGAGACCGCAGAGGAACAGGGCTACACATCAGCCCTCGTAGAGCTTGAGGGC
  GATAATGCGCGGCTCGCAGTGCTTAAGCGGTCTGTGGAGCTGACCCACTTCGCCTATCCCGCTGTTAATC
  TGCCCCCAAAGGTTATGCGCCGGATTATGGACCAGCTCATTATGCCTCATATCGAAGCTCTCGATGAGAG
  TCAGAAACAGAGACCGGAGGATGCCCAGCCAGTCGTTAGCGATGAAATGCTGGCCCGCTGGCTGGGAAAC
  CGGGATCCACAATCCTTGGAACAGCGGCGAAAGCTGATGCTCGCTGTCGTGCTTGTGACACTCGAGCTGG
  AGTGTATGCGGCGCTTCTTCTCTGATCCGGAGACTCTTAGAAAGGTGGAAGAAACTCTCCATTATACCTT
  CCGGCATGGTTTCGTCCGGCAAGCCTGCAAGATATCCAACGTCGAACTGACAAATTTGGTGTCATGTCTG
  GGGATCCTGCACGAGAACCGGCTCGGACAGACAGTGCTTCATTCCACGCTCAAGGGTGAAGCTAGAAGGG
  ACTACATACGCGACTGTGTGTTTCTGTTCCTGTGTCATACATGGCAGTCAGCCATGGGAGTGTGGCAGCA
  GTGCCTCGAAGACGAGAATCTGAAGGAACTGGACAAGCTCTTGGCTAAGAACCTGAAAACCTTGTGGACC
  GGGTTCGATGAACGCACAGTGGCTTTGGACCTGGCCGAGATCGTGTTCCCAGAAAGACTGCGGCAGACTC
  TGAAGGGTGGGCTCCCGGATTTCATGTCTCAATCCATGCTGCAGAATTACCGGACATTTATCCTGGAGCG
  CTCCGGGATCCTCCCTGCCACCTGTAACGCTTTTCCCTCCGACTTCGTCCCACTGAGCTACCGGGAATGC
  CCCCCGCCCTTGTGGTCCCACTGCTATCTGCTGCAGCTTGCGAACTACATTGCCTACCACTCAGATGTGA
  TCGAGGACGTTTCCGGCGAGGGGCTGCTGGAGTGCCACTGTAGATGCAATTTGTGCAGCCCACATAGAAG
  TCTGGTATGTAATCCTCAGTTGTTGTCTGAGACCCAAGTCATTGGCACGTTTGAACTCCAGGGTCCCGAA
  AAGTCAACAGCACCGCTTAAACTGACACCAGGGCTTTGGACGTCTGCTTATCTCCGGAAGTTTGTTCCAC
  AGGACTATCACGCACACGAAATCAAATTCTTTGAAGATCAGCTGAGGCCACAGCACGCGGACCTCACCGC
  TTGCGTTATTACGCAGGGTACGATCCTGGCCCAGCTTCACGCAATCCAGAAGTCCCGGCAGGAATTTCTC
  CTGAAAAAGGGCAAGGGAGTATACCTGGATCCCCAGACAGGAGAAGTCCTGAATCCTGGACTGCCCCAGC
  ATGCCAAAAAGGAGGCCGGCGCTACCAGCGGTGGAGATGGAAGACGAATGGGACAGCCCGGTAGAGGTGG
  TCGGATGGGTGGTGGACGGATCGGCCGGGGGGGCACAGGAGCGGGCAATAGGGCTGCGCGAAGACGAACA
  ATCAGAGCTGGATCACCTAGTGGCCAGGCCTCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100164 representing YP_003038615
  Red=Cloning sites Green=Tags
  
  MEEQPRKQEQEEENLTTHEQPKIKQDLGLEEPARLEPPQDEQKETNAGLEHGYLGGQEDVLLKHLQRQSF
  ILRDALADRSKTPLSVQELCRAYELNLFSPRVPPKRQPNGTCEPNPRLNFYPVFAVPEALATYHIFFKNQ
  KIPVSCRANRTRADALLALGPGARIPDIASLEEVPKIFEGLGRDETRAANALKETAEEQGYTSALVELEG
  DNARLAVLKRSVELTHFAYPAVNLPPKVMRRIMDQLIMPHIEALDESQKQRPEDAQPVVSDEMLARWLGN
  RDPQSLEQRRKLMLAVVLVTLELECMRRFFSDPETLRKVEETLHYTFRHGFVRQACKISNVELTNLVSCL
  GILHENRLGQTVLHSTLKGEARRDYIRDCVFLFLCHTWQSAMGVWQQCLEDENLKELDKLLAKNLKTLWT
  GFDERTVALDLAEIVFPERLRQTLKGGLPDFMSQSMLQNYRTFILERSGILPATCNAFPSDFVPLSYREC
  PPPLWSHCYLLQLANYIAYHSDVIEDVSGEGLLECHCRCNLCSPHRSLVCNPQLLSETQVIGTFELQGPE
  KSTAPLKLTPGLWTSAYLRKFVPQDYHAHEIKFFEDQLRPQHADLTACVITQGTILAQLHAIQKSRQEFL
  LKKGKGVYLDPQTGEVLNPGLPQHAKKEAGATSGGDGRRMGQPGRGGRMGGGRIGRGGTGAGNRAARRRT
  IRAGSPSGQASS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 2136 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038615
• RefSeq ORF: 2136
• MW: 80.2 kDa