hexon (NC_012959) Virus Tagged ORF Clone

CAT#: VC100161

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus 54], codon optimized for human cell expression, YP_003038612

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hexon
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100161 represents NCBI reference of YP_003038612 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCCTTACCGCCAGAGAGAGAGCCAAGATGGCTACTCCATCAATGATGCCCCAGTGGGCTTACATGC
  ACATCGCCGGACAGGACGCCTCAGAATACCTGTCTCCCGGGCTCGTCCAGTTTGCCCGAGCGACAGATAC
  TTACTTTTCCCTCGGAAATAAGTTCCGCAATCCTACCGTTGCTCCCACTCACGATGTGACAACTGACCGC
  TCACAGCGGCTGACCCTGCGATTCGTGCCCGTGGATAGAGAAGACACAACTTACAGTTACAAGGCCCGCT
  TTACCCTCGCCGTGGGCGATAACCGCGTGCTTGATATGGCCAGTACCTATTTTGATATCAGGGGCGTGCT
  GGACCGAGGACCCAGCTTTAAACCCTATAGTGGGACTGCCTATAATTCTTTGGCGCCAAAGGGGGCTCCA
  AATTCCAGCCAATGGATCACTAAGCAGACCAATGCCGGTAACGAAACAACGAAAACCCACACGTACGGCG
  TAGCCGCCATGGGCGGTGCAGATATCACCATTAAGGGGCTTCAAATCGGCGTCGATAAGACTGAGAATAA
  AAATGAGCCTATTTACGCAAACGAAATATATCAGCCCGAACCACAGGTCGGTGAAGAGAACCTGCAGGAT
  GTCGAGAACTACTACGGCGGACGGGCATTGAAGAAGGAAACAAAAATGAAACCATGCTACGGCAGTTTCG
  CCAGACCCACAAACGAGAAGGGCGGACAAGCTGTGTTCAAGACCGGCAACGACGGACAGCCAACTACAGA
  GCATGATATCACCATGGCATTTTTCGACACCCCAGGCGACACTAACGCCGAAGATACGGAACTTGAGGCC
  GATATCGTTATGTATACGGAAAACGTAAATCTCGAGACACCAGATACACACGTGGTGTATAAACCTGGAC
  CACTGGAGGACTCCTCCGAAATTAATCTTACGCAGCAATCTATGCCCAATCGCCCTAACTACATCGGATT
  TCGCGACAACTTCGTGGGCCTCATGTACTACAACAGCACCGGCAATATGGGTGTGCTGGCCGGCCAGGCC
  AGCCAGCTGAATGCCGTTGTGGATCTGCAAGATAGAAATACAGAGCTCTCCTACCAGCTTCTGCTTGACT
  CACTGGGAGACAGGACAAGGTATTTTTCCATGTGGAATTCCGCCGTGGACTCATACGATCCGGATGTGAG
  AATCATCGAAAACCACGGCGTTGAGGACGAGCTCCCGAATTATTGTTTCCCCCTGGATGGCGCCGGGACC
  AACGCCACCTACCAGGGCGTGAAGGTTAAGAACGGGCAGGACGGCGATAATAACGCAGAATGGGAGAAGG
  ACAACGCTGTGGCTGACCGGAACCAGATTTGTAAGGGAAACATCGTGGCCATGGAAATCAACCTGCAGGC
  CAATCTGTGGAAGAGTTTCTTGTATTCTAACGTCGCACTTTACCTCCCTGATAGCTTTAAATATACTCCT
  GCAAACGTGACTCTTCCTACCAATACTAATACATACGAGTACATGAACGGCCGAGTCGTGGCTCCCAGCC
  TCGTGGACGCCTACGTGAATATCGGTGCCCGCTGGTCCTTGGACCCTATGGATAATGTAAACCCCTTCAA
  CCACCATAGGAATGCCGGCCTCCGATACAGATCTATGCTCCTGGGCAACGGGAGGTACGTACCGTTCCAT
  ATACAGGTCCCGCAGAAGTTTTTTGCCATTAAAAACCTGTTGCTGCTTCCGGGCTCTTATACCTACGAAT
  GGAACTTCCGCAAGGATGTGAACATGATACTGCAGTCTAGTCTTGGAAATGATCTTCGGGTTGATGGTGC
  ATCTGTCAGATTCGACTCAGTGAACCTGTACGCGACCTTTTTCCCTATGGCACACAACACTGCCAGTACC
  CTCGAGGCCATGCTGCGGAACGACACCAATGACCAGTCCTTTAACGACTACCTGTCAGCTGCAAACATGC
  TCTACCCCATTCCGGCCAAAGCCACCAATGTACCCATCAGTATCCCATCACGCAATTGGGCGGCTTTTCG
  AGGGTGGAGCTTCACTAGGCTCAAGACCAAAGAGACGCCTAGCCTGGGCTCAGGGTTCGACCCTTATTTC
  GTCTATAGCGGCTCCATACCATACTTGGACGGAACCTTTTATCTGAACCACACTTTTAAGAAAGTAAGCA
  TCATGTTCGATAGCTCCGTGTCCTGGCCCGGCAACGATCGCTTGCTTACCCCTAACGAATTCGAGATCAA
  GCGGTCTGTGGACGGAGAAGGCTATAACGTCGCTCAGTGTAATATGACCAAGGACTGGTTTCTCATTCAG
  ATGCTCTCTCATTATAATATTGGTTATCAGGGGTTTCACGTGCCAGAGGGTTATAAAGACCGAATGTATA
  GCTTCTTCAGAAACTTCCAGCCAATGAGCAGGCAGGTGGTGGACGAAATTAATTATAAGGACTACAAAGC
  GGTAGCGTTGCCGTTCCAACATAACAATTCAGGCTTCACCGGCTACCTGGCTCCCACGATGCGACAGGGC
  CAGCCATACCCGGCAAACTTCCCGTACCCACTGATAGGGGAGACCGCCGTACCCTCAGTGACTCAGAAGA
  AATTTTTGTGCGACCGCGTGATGTGGCGAATCCCATTTAGTTCCAATTTCATGTCCATGGGAGCTCTCAC
  CGACCTGGGGCAGAACATGCTTTACGCGAATAGTGCACACGCCTTGGATATGACTTTTGAAGTGGACCCA
  ATGGATGAGCCTACTCTCCTGTACCTCCTCTTCGAGGTGTTTGACGTCGTCCGGGTCCACCAACCTCACC
  GAGGGGTCATCGAGGCTGTCTACCTCCGCACTCCATTCTCTGCGGGCAATGCCACCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100161 representing YP_003038612
  Red=Cloning sites Green=Tags
  
  MCLTARERAKMATPSMMPQWAYMHIAGQDASEYLSPGLVQFARATDTYFSLGNKFRNPTVAPTHDVTTDR
  SQRLTLRFVPVDREDTTYSYKARFTLAVGDNRVLDMASTYFDIRGVLDRGPSFKPYSGTAYNSLAPKGAP
  NSSQWITKQTNAGNETTKTHTYGVAAMGGADITIKGLQIGVDKTENKNEPIYANEIYQPEPQVGEENLQD
  VENYYGGRALKKETKMKPCYGSFARPTNEKGGQAVFKTGNDGQPTTEHDITMAFFDTPGDTNAEDTELEA
  DIVMYTENVNLETPDTHVVYKPGPLEDSSEINLTQQSMPNRPNYIGFRDNFVGLMYYNSTGNMGVLAGQA
  SQLNAVVDLQDRNTELSYQLLLDSLGDRTRYFSMWNSAVDSYDPDVRIIENHGVEDELPNYCFPLDGAGT
  NATYQGVKVKNGQDGDNNAEWEKDNAVADRNQICKGNIVAMEINLQANLWKSFLYSNVALYLPDSFKYTP
  ANVTLPTNTNTYEYMNGRVVAPSLVDAYVNIGARWSLDPMDNVNPFNHHRNAGLRYRSMLLGNGRYVPFH
  IQVPQKFFAIKNLLLLPGSYTYEWNFRKDVNMILQSSLGNDLRVDGASVRFDSVNLYATFFPMAHNTAST
  LEAMLRNDTNDQSFNDYLSAANMLYPIPAKATNVPISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYF
  VYSGSIPYLDGTFYLNHTFKKVSIMFDSSVSWPGNDRLLTPNEFEIKRSVDGEGYNVAQCNMTKDWFLIQ
  MLSHYNIGYQGFHVPEGYKDRMYSFFRNFQPMSRQVVDEINYKDYKAVALPFQHNNSGFTGYLAPTMRQG
  QPYPANFPYPLIGETAVPSVTQKKFLCDRVMWRIPFSSNFMSMGALTDLGQNMLYANSAHALDMTFEVDP
  MDEPTLLYLLFEVFDVVRVHQPHRGVIEAVYLRTPFSAGNATT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 2859 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038612
• RefSeq ORF: 2859
• MW: 107.4 kDa