DNA binding protein (NC_012959) Virus Tagged ORF Clone

CAT#: VC100163

Myc-DDK-tagged ORF clone of viral ORF for DNA binding protein [Human adenovirus 54], codon optimized for human cell expression, YP_003038614

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DNA binding protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100163 represents NCBI reference of YP_003038614 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGGGTAGTCAGGACGTTAGAAGGTTCATGGACAGGGAAGCAACACCACCACGGGGACACGGGA
  GCGCCAGGTATCCCCCTGAACAGGAGAGATCCCCATCACCACCACTCCCACCTCTCCCTACTAAACGGCG
  CAAGTATCAAAGGGTGGGCTCTGGATCTTCCGAGGAGGACGTAGTGCCCCTGGACAGTCCCCCCAAAAAG
  AAACAGCCTCGGAAGACCAAGCATGTGACGAAAGTGAACCCCGATGAAGAGATGCCCCAGGAGGACGCTG
  TGATCGTTGGGGTCGGTTTTAGCCAGCCTCCCGTGCTGTTGAAGGAAGGCAAGGATGGCAAGCGGATAGT
  CCAGCCCGCCACCCCCGGCATTCTGAATGTTTGTAATCCACTGTCCTTGCCCCTGGTGTCAAGCTGGGAA
  AAGGGTATGGACACAATGAATGTGCTCATGGAACGATATAGAGTGGATTCCGGGCTGAGGGACGCCTATA
  AGCTTATGCCGGAACAGACAGAGATTTTTCAGAAGATGTGTCAGACATGGATAAACGAAGAGGCACGGGG
  AATGCAGCTGACGTTCACCACCCAAAAAAGTTTCTCAACAGTTATGGGGCGGCTGCTCCAAGGCTATATT
  TTTTCTCACTCAGGTATCGCCCATAAGAACTGGGAGTGCACCGGTAGCGCTCTGTGGGATCACGGATGCG
  CCGAGGTGGAGGGCCAGCTGAAGTGTCTCCATGGCACCGTGATGATCCATAAAGATCACGTAGTGGAAAT
  GGACGTTACAAGTGAAAATGGGCAGCGCGCCCTCAAGGAACAGCCCTCCAAGGCTAAGGTGACTCAGAAC
  CGCTGGGGGAGGTCAGTGGTCCAGCTCACCTCCCACGACGCCCGCTGCTGTGTGCAGGATGCAGGGTGCG
  GTAACAATCAGTTTTCCGGTAAAAGCTGCGGGCTGTTCTTCTCCGAAGGCGCTAAAGCACAACAGGCTTT
  TAAACAGATCATGGCCTTCATTAAGGCGCTGTACCCAAATATGCAGCGCGGCGCCGGCATGATGCTCATG
  CCTGTGCATTGTGAGTGCAACCATAAGCCTCACAGCGTGCCCTTCCTTGGAAGACAGCTGTGCAAGATGA
  CCCCCTTCGGGCTTAGCAACGCAGAAGACCTTGACAAAGACCAGATAAGTGACAAATCCGTGCTGGCATC
  CGTGAAGTATCCCTCCCTGATGGTCTTCCAGTGCTGCAACCCGGTCTACCGCAATTCCCGGGCTCAAAGT
  ACGGGGCCTAATTGCGACTTCAAGATCAGCGCCCCCGACATGCTCGGGGCTCTGCAGATGAGCCGGAGAA
  TGTGGAGCGAGACCTTCCCGGAAATCGCGGTTCCTAAGCTCGTTATTCCCGAATTCAAGTGGCAACCAAA
  ATACCAGTACAGGAACGTCGCCCTCCCCTCCGCTGCCCACAACGATGAGCGGGAGAATCCTTTCGATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100163 representing YP_003038614
  Red=Cloning sites Green=Tags
  
  MAGGSQDVRRFMDREATPPRGHGSARYPPEQERSPSPPLPPLPTKRRKYQRVGSGSSEEDVVPLDSPPKK
  KQPRKTKHVTKVNPDEEMPQEDAVIVGVGFSQPPVLLKEGKDGKRIVQPATPGILNVCNPLSLPLVSSWE
  KGMDTMNVLMERYRVDSGLRDAYKLMPEQTEIFQKMCQTWINEEARGMQLTFTTQKSFSTVMGRLLQGYI
  FSHSGIAHKNWECTGSALWDHGCAEVEGQLKCLHGTVMIHKDHVVEMDVTSENGQRALKEQPSKAKVTQN
  RWGRSVVQLTSHDARCCVQDAGCGNNQFSGKSCGLFFSEGAKAQQAFKQIMAFIKALYPNMQRGAGMMLM
  PVHCECNHKPHSVPFLGRQLCKMTPFGLSNAEDLDKDQISDKSVLASVKYPSLMVFQCCNPVYRNSRAQS
  TGPNCDFKISAPDMLGALQMSRRMWSETFPEIAVPKLVIPEFKWQPKYQYRNVALPSAAHNDERENPFDF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038614
• RefSeq ORF: 1470
• MW: 54.9 kDa