pVIII (NC_012959) Virus Tagged ORF Clone

CAT#: VC100166

Myc-DDK-tagged ORF clone of viral ORF for pVIII [Human adenovirus 54], codon optimized for human cell expression, YP_003038617

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100166 represents NCBI reference of YP_003038617 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAAGGAAATTCCCACACCATATATGTGGAGTTATCAGCCTCAGATGGGTCTTGCCGCGGGCGCAT
  CCCAGGACTACTCAACACGGATGAACTGGCTGTCTGCCGGCCCCTCTATGATTTCTCAGGTGAACGGCAT
  TAGGAATCACAGAAACCAAATCCTTCTCGAACAGGCAGCCGTAACCTCCACACCGCGCGCAAAATTGAAT
  CCGCGCAATTGGCCGAGTACATTGGTTTATCAGAAAATCCCAGGCCCGACAACAGTGCTTCTGCCACGAG
  ATGCACTGGCGGAGGTGCGAATGACCAATTCCGGTGTTCAGCTGGCAGGTGGCGCGAGCAGGTGTCCCCC
  GAGGCCAGACCTGGGAATCAAGACCCTCCTGATCCGCGGTAAAGGTATACAGCTGAACAATGAACTGGTG
  TCCAGTTCCATTGGGCTGAGACCTGATGGTGTGTTTCAACTGGCCGGGGCCGGGAAAAGCAGCTTCACGC
  CTAACCAGGCCTATCTGACACTCCAGTCATCTTCCTCCCAGCCCCGCTCTGGAGGCATTGGCACACTTCA
  GTTTGTGGAGGAGTTTGTGCCATCCGTCTATTTCAATCCCTTTAGTGGATCTCCCGGGCTTTATCCCGAC
  GAGTTTATCCCCAACTTTGACGCCGTGCGGGAGGCCGTGGATGGTTATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100166 representing YP_003038617
  Red=Cloning sites Green=Tags
  
  MSKEIPTPYMWSYQPQMGLAAGASQDYSTRMNWLSAGPSMISQVNGIRNHRNQILLEQAAVTSTPRAKLN
  PRNWPSTLVYQKIPGPTTVLLPRDALAEVRMTNSGVQLAGGASRCPPRPDLGIKTLLIRGKGIQLNNELV
  SSSIGLRPDGVFQLAGAGKSSFTPNQAYLTLQSSSSQPRSGGIGTLQFVEEFVPSVYFNPFSGSPGLYPD
  EFIPNFDAVREAVDGYD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038617
• RefSeq ORF: 681
• MW: 24.6 kDa