pVIII (NC_012959) Virus Tagged ORF Clone

CAT#: VC100166

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for pVIII [Human adenovirus 54], codon optimized for human cell expression, YP_003038617


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Reconstitution Protocol

USD 400.00

3 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol pVIII
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100166 represents NCBI reference of YP_003038617 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTAAGGAAATTCCCACACCATATATGTGGAGTTATCAGCCTCAGATGGGTCTTGCCGCGGGCGCAT
CCCAGGACTACTCAACACGGATGAACTGGCTGTCTGCCGGCCCCTCTATGATTTCTCAGGTGAACGGCAT
TAGGAATCACAGAAACCAAATCCTTCTCGAACAGGCAGCCGTAACCTCCACACCGCGCGCAAAATTGAAT
CCGCGCAATTGGCCGAGTACATTGGTTTATCAGAAAATCCCAGGCCCGACAACAGTGCTTCTGCCACGAG
ATGCACTGGCGGAGGTGCGAATGACCAATTCCGGTGTTCAGCTGGCAGGTGGCGCGAGCAGGTGTCCCCC
GAGGCCAGACCTGGGAATCAAGACCCTCCTGATCCGCGGTAAAGGTATACAGCTGAACAATGAACTGGTG
TCCAGTTCCATTGGGCTGAGACCTGATGGTGTGTTTCAACTGGCCGGGGCCGGGAAAAGCAGCTTCACGC
CTAACCAGGCCTATCTGACACTCCAGTCATCTTCCTCCCAGCCCCGCTCTGGAGGCATTGGCACACTTCA
GTTTGTGGAGGAGTTTGTGCCATCCGTCTATTTCAATCCCTTTAGTGGATCTCCCGGGCTTTATCCCGAC
GAGTTTATCCCCAACTTTGACGCCGTGCGGGAGGCCGTGGATGGTTATGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100166 representing YP_003038617
Red=Cloning sites Green=Tags

MSKEIPTPYMWSYQPQMGLAAGASQDYSTRMNWLSAGPSMISQVNGIRNHRNQILLEQAAVTSTPRAKLN
PRNWPSTLVYQKIPGPTTVLLPRDALAEVRMTNSGVQLAGGASRCPPRPDLGIKTLLIRGKGIQLNNELV
SSSIGLRPDGVFQLAGAGKSSFTPNQAYLTLQSSSSQPRSGGIGTLQFVEEFVPSVYFNPFSGSPGLYPD
EFIPNFDAVREAVDGYD

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_012959
ORF Size 681 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_012959.1, YP_003038617
RefSeq ORF 681
MW 24.6 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.