44.5 kDa protein (NC_012959) Virus Tagged ORF Clone

CAT#: VC100170

Myc-DDK-tagged ORF clone of viral ORF for 445 kDa protein [Human adenovirus 54], codon optimized for human cell expression, YP_003038621

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 44.5 kDa protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100170 represents NCBI reference of YP_003038621 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATGTCAAGACGCAAGAACTGCTATAAGTTTTTCTTTTTCTTCTATTCCACAATGAATACACTCA
  CTTCCGTTGTCCTTCTTTCTCTTCTGGTCGCCTTCTCCGAGGCTGGAATCATCAATCTTAACGTCTCATG
  GGGCATCAATCTTACTCTTGTCGGCCCGCTGGACCTCCCAGTCACCTGGTATGACGGCAAAGGAATGCAG
  TTCTGTGACGGGAACACGATCAAAAATCCCCAAATAAAGCATTCTTGCAACCAACAGAATCTTACCTTGC
  TCAATGCTGACAAGAGCCATGAAAGAACCTATCTGGGATACCGACATGACAGCAAAGGCAAGGTTGACTA
  CAAAGTTACTGTCATTCCCCCTCCTTCCACAACCCGAAAGCCCCTCTCACAGCCACATTACGTTACCGTT
  ACAATGAACCACAACATGACCCTTGTGGGTCCCCTGAACTTGCCTGTGACCTGGTACGATGGGGAGGGAA
  ATAAGCTGTGCAATGGAGAGAAGGTCGAGCACGCCGAATTCAACCACACCTGCAACATACAAAATCTCAC
  CTTGCTCTTCGTGAACCTGACTCACAATGGGGCCTATATTGGTTACAATAAAGACGGGAGTAACCGCGAA
  CTGTATGAGGTCTCTGTGAAAACCCTGTTTCAGAATGGCGCCAAACAGTCAAAAGTAGAGCAGTCTAATA
  TAGGACAAGGAAATACAGCCCAATCCGCCAAGAGGAAATCAACCAATAACCTGCAGCCTACCCAGCTGTA
  TGTGCGGCCTTTCACAAACGTGAGCCTGACAGGACCACCCAACGGAAAAGTGATTTGGTACGACGGCGAG
  CTGAATGACCCTTGCGAGCAGAAATATAAACTGAAAACCTTCTGCAACCAGCAGAATCTGACACTTATTA
  ACGTTACTTCCACATACGACGGCATCTACTACGGAACCGACGAAAAGGACAAAGCCAATAGGTACCGGAT
  TAAAGTTAACACAACCAATCACAAGACGGTAAAGATCAAACCACACACGAAGGAGCCACCGGCTGAGCAG
  AAGAAACAATTCCAACTTCAGGTGGCTGAGACCGATCAGAATGAGAGCAAAATTCCATCCGCTACAGTCG
  CTATCGTTGTGGGAGTTATCGCAGGATTTGTGACCCTGATCATCGTATTTATATGCTACATTTGTTGTCG
  GAAGCGGCCCCGGGCCTACAATTACATGGTCGACCCACTGCTTTCTTTCTCCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100170 representing YP_003038621
  Red=Cloning sites Green=Tags
  
  MEMSRRKNCYKFFFFFYSTMNTLTSVVLLSLLVAFSEAGIINLNVSWGINLTLVGPLDLPVTWYDGKGMQ
  FCDGNTIKNPQIKHSCNQQNLTLLNADKSHERTYLGYRHDSKGKVDYKVTVIPPPSTTRKPLSQPHYVTV
  TMNHNMTLVGPLNLPVTWYDGEGNKLCNGEKVEHAEFNHTCNIQNLTLLFVNLTHNGAYIGYNKDGSNRE
  LYEVSVKTLFQNGAKQSKVEQSNIGQGNTAQSAKRKSTNNLQPTQLYVRPFTNVSLTGPPNGKVIWYDGE
  LNDPCEQKYKLKTFCNQQNLTLINVTSTYDGIYYGTDEKDKANRYRIKVNTTNHKTVKIKPHTKEPPAEQ
  KKQFQLQVAETDQNESKIPSATVAIVVGVIAGFVTLIIVFICYICCRKRPRAYNYMVDPLLSFSY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038621
• RefSeq ORF: 1245
• MW: 47.0 kDa