pIVa2 (NC_012959) Virus Tagged ORF Clone

CAT#: VC100151

Myc-DDK-tagged ORF clone of viral ORF for pIVa2 [Human adenovirus 54], codon optimized for human cell expression, YP_003038602

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pIVa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100151 represents NCBI reference of YP_003038602 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACTAGAGGGAGGCGCCCATGTCCTTTTCAGCACCAGCAGGATGAGTCCGAGGCCCACACCTGTA
  AACGCCCCGCCCGCGGTCCGCCAATACACAGGAATGGCGATCACCCCCACGCCGACCCAGAGACTCTGGA
  AGGTCATGACGCCGGAAGGGCCGGTCGCCCCAGTTCATGCGCCCTGCAGTCCCAAAGCTCACAGCCCCCA
  AAAAGGGGCTCTCTGCTGGATCCCGATGCAGTCGAACACGTTACCGAACTTTGGGATAGGCTTGAACTTC
  TGAGCCAAACCCTGGCCAAAATGCCAATGGCAGATGGCCTTAAACCTCTGAAGAACTTCGCGTCTCTCCA
  GGAGTTGCTTTCCCTCGGTGGGGACAGGCTCCTTAGTGAGCTCGTCAGGGAGAACCTGCAGGTGAGGGAC
  ATGCTGAACCAAGTGGCCCCGTTGCTGAGGGATGACGGGTCATGCATCTCCCTCAATTATCATTTGCAAC
  CAGTGATAGGAGTTATTTACGGACCTACCGGTTGCGGGAAGAGCCAACTGCTGAGGAACCTGTTGAGCTC
  TCAACTTATCACACCGGCTCCCGAGACCATCTTCTTCATCGCACCACAGGTGGATATGATCCCACCCAGC
  GAGATGAAAGCCTGGGAGATGCAAATTTGTGAGGGCAACTTCGCTCCTGGCCCTGAGGGCACCATAGTTC
  CCCAGTCCGGGACCCTGCGCCCCAAATTTATCAAGATGAGCTACGACGACTTGACACAGGAGCACAATTA
  TGATGTATCAGATCCCAGAAATGTTTTTGCCAAAGCCGCAGCTCACGGGCCTATAGCGATTATCATGGAC
  GAATGTATGGAAAATCTTGGAGGACATAAAGGTGTGTCTAAGTTTTTCCACGCTTTCCCATCTAAACTCC
  ATGACAAGTTTCCAAAGTGTACCGGTTACATTGTTTTGGTGGTTCTGCATAACATGAATCCGCGCCGGGA
  CCTCGGAGGAAACATTGCCAACCTGAAGATTCAAGCCAAACTCCACATTATATCCCCTCGGATGCATCCA
  TCACAGCTCAACCGGTTCGCAAATACTTATACCAAGGGATTGCCAGTGGCCATCTCACTCCTTTTGAAAG
  ACATTATTCAACACCATGCCCAAAGGCCCTGCTATGATTGGATCATTTATAACACAACGCCAGAGCATGA
  AGCTATGCAGTGGTGCTATCTGCATCCAAGAGATGGGCTCATGCCCATGTACCTGAACATTCAATCCCAC
  CTCTATAGAGTGCTTGAGAAGATCCACAGAACTCTCAATGATAGAGAACGGTGGACCAGGGCCTATCGGG
  CCAGGAAGAACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100151 representing YP_003038602
  Red=Cloning sites Green=Tags
  
  METRGRRPCPFQHQQDESEAHTCKRPARGPPIHRNGDHPHADPETLEGHDAGRAGRPSSCALQSQSSQPP
  KRGSLLDPDAVEHVTELWDRLELLSQTLAKMPMADGLKPLKNFASLQELLSLGGDRLLSELVRENLQVRD
  MLNQVAPLLRDDGSCISLNYHLQPVIGVIYGPTGCGKSQLLRNLLSSQLITPAPETIFFIAPQVDMIPPS
  EMKAWEMQICEGNFAPGPEGTIVPQSGTLRPKFIKMSYDDLTQEHNYDVSDPRNVFAKAAAHGPIAIIMD
  ECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYIVLVVLHNMNPRRDLGGNIANLKIQAKLHIISPRMHP
  SQLNRFANTYTKGLPVAISLLLKDIIQHHAQRPCYDWIIYNTTPEHEAMQWCYLHPRDGLMPMYLNIQSH
  LYRVLEKIHRTLNDRERWTRAYRARKNK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038602
• RefSeq ORF: 1344
• MW: 50.7 kDa