20.9 kDa protein (NC_012959) Virus Tagged ORF Clone

CAT#: VC100168

Myc-DDK-tagged ORF clone of viral ORF for 209 kDa protein [Human adenovirus 54], codon optimized for human cell expression, YP_003038619

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 20.9 kDa protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100168 represents NCBI reference of YP_003038619 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCATTTTCGCCGTTCTGTTTGTGGTTAGCCTGATCAAAGCCGAAATCCGCCTCTACAGCGGGATCC
  AGTGCCGACATAATAGAACCAAAACGATTAACTTCACTACTCAAGAACAAGTGAATTTTACTTGTAAGCC
  ACATAAAAAGTATCTGATATGGTTTCTCAAGAACACCACTTTCGCAGTCGTCAATACTTGCGACAATGAT
  GGCGTCCTCCTTCCCAACAATCTGACCAGCGGTCTTGCTTTCTCCGTGAAACGCGCTAAACTGATACTGC
  ACCGACCTATTGTGGAGGGCACATATCACTGTCAGAGCGGTCCCTGTCACCACATATTCCACCTCGTGAA
  CGTGACTAGTTCCAGCAATAGCAGCGAGACGAATCTTTCTTCAAGGACCAATCGACCCCAATTTGGAGGA
  GAGCTCCGCCTCCCACCATGTAAGGAGGGAGTGTCACCATACAAAGTCGTGGGCTATTTGATCCTCGGGG
  CTGTTCTTGGAGGATGCATCGCCGTGCTGGCCCAGCTCCCTTGTTGGGTGGAGATCAATCTGTACCTGCT
  GGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100168 representing YP_003038619
  Red=Cloning sites Green=Tags
  
  MRIFAVLFVVSLIKAEIRLYSGIQCRHNRTKTINFTTQEQVNFTCKPHKKYLIWFLKNTTFAVVNTCDND
  GVLLPNNLTSGLAFSVKRAKLILHRPIVEGTYHCQSGPCHHIFHLVNVTSSSNSSETNLSSRTNRPQFGG
  ELRLPPCKEGVSPYKVVGYLILGAVLGGCIAVLAQLPCWVEINLYLLG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 564 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038619
• RefSeq ORF: 564
• MW: 21.0 kDa