pVI (NC_012959) Virus Tagged ORF Clone

CAT#: VC100160

Myc-DDK-tagged ORF clone of viral ORF for pVI [Human adenovirus 54], codon optimized for human cell expression, YP_003038611

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100160 represents NCBI reference of YP_003038611 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATATCAATTTCGCCTCTCTGGCTCCACGGCATGGTACAAGGCCGTTCATGGGGACTTGGAATG
  AGATCGGGACCTCTCAGCTTAACGGCGGCGCTTTTAACTGGAGCAGCGTATGGTCCGGACTTAAAAATTT
  CGGGAGCACTTTGCGGACCTACGGAAATAAAGCTTGGAATAGCAGCACCGGCCAGATGCTTAGAGAGAAA
  CTCGCTGACCAGAATTTCCAGCAGAAGGTCGTAGACGGGTTGGCCAGCGGTATTAACGGTGTTGTAGACA
  TTGCCAACCAGGCCGTGCAACGAGAAATTAACTCACGCCTGAATCCAAGGCCACCCACCGTGGTGGAGAT
  GGAGGACGCCACACCCCCTCCACGAGGGGAGAAACGCCCCCGGCCAGATGCCGAAGAAACGACCTTGCAG
  GTAGACGAACCCCCGAGCTACGAAGAGGCTCTCAAAGCTGGGATGCCTACCACGCGGATTATAGCCCCAA
  TGGCCACAGGCGTGATGAAACCCGCTACACTCGATCTCCCATCTCCTCCTGTTCCTGCTCCTCCTAAGGC
  CGCCCCGGTGGTTCAGGCCCCACCCGTGGCGACAGCTGTGCGAAGGGTGCCCGCTAGGAGGCAAGCTCAG
  AACTGGCAGTCCACCCTGCACTCTATCGTGGGCCTTGGAGTTAAATCTCTGAAACGCCGCCGATGCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100160 representing YP_003038611
  Red=Cloning sites Green=Tags
  
  MEDINFASLAPRHGTRPFMGTWNEIGTSQLNGGAFNWSSVWSGLKNFGSTLRTYGNKAWNSSTGQMLREK
  LADQNFQQKVVDGLASGINGVVDIANQAVQREINSRLNPRPPTVVEMEDATPPPRGEKRPRPDAEETTLQ
  VDEPPSYEEALKAGMPTTRIIAPMATGVMKPATLDLPSPPVPAPPKAAPVVQAPPVATAVRRVPARRQAQ
  NWQSTLHSIVGLGVKSLKRRRCY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 699 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038611
• RefSeq ORF: 699
• MW: 25.4 kDa