E1B 55 kDa protein (NC_012959) Virus Tagged ORF Clone

CAT#: VC100149

Myc-DDK-tagged ORF clone of viral ORF for E1B 55 kDa protein [Human adenovirus 54], codon optimized for human cell expression, YP_003038600

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B 55 kDa protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100149 represents NCBI reference of YP_003038600 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGGACACCCGACCAAGCAGGGTCTGCACAGCGGTTTGTGTTCACACGCCCCGGTGGAGGGCT
  TGGACGAAGCAGCCGGAACAGAAAATCTGGAATTGCTGGCCTTTACAGCTAGCAGTGTCGGCAGCTCTAG
  CAGTACCCAAACCAACATCCACGTCGGCGGAAGAAACGAGGGCGGACACGGTCGCGAACCCGAGGAAAGA
  CCCGGACCTTCCGTCGGACGGGGAGCTGGCCTGAATGAGGTGTCTTCACTCTACCCCGAGCTGTCTAAGG
  TTCTTACTTCCATGGCTAGAGGAGTAAAACGGGAACGCAGCGATGGGGGAAACACTGGTCTCATGACGGA
  ACTCACAGCTTCACTCATGAACCGGAAACGCCCAGAACGCATTACATGGCATGAACTCCAGCAAGAGTGT
  CGGGATGAAATTGGACTGATGCAGGACAAGTATGGCCTTGAGCAAATTAAGACCCATTGGCTGAATCCCG
  ATGAAGATTGGGAGGAGGCGATCAAGAAATATGCGAAAATCGCTCTGAGACCGGATTGTAAGTACCGGGT
  CACAAAAACCGTGAATATCAAGCATGCCTGTTATATCTCCGGTAATGGGGCTGAGGTTGACATAGACACC
  CTCGACAAATCCGCCTTCAGATGTTGCATGATGGGGATGCGGGTGGGGGTGATGAACATGAACTCAATGA
  TCTTCATTAACATAAAATTCAACGGCGAGAAATTTAACGGCGTCCTGTTTATGGCAAATTCCCATATGAC
  GCTGCATGGCTGCAGCTTCTTCGGCTTTAATAACATGTGTGCAGAGGTCTGGGGGGCAGCTAAAATTAGA
  GGCTGCAAGTTCTATGGGTGTTGGATGGGCGTAGTGGGAAGACCCAAATCCGAGATGTCTGTCAAACAGT
  GCGTGTTTGAAAAATGTTACCTCGGGGTTTGCACAGAGGGCAATGCCCGGGTGAGACACTGTTCTTCTCT
  GGAGACGGGCTGCTTCTGTCTGGTAAAAGGGACCGCCAGCATTAAGCACAACGTCGTTAAAGGCTGTACG
  GACGAAAGAATGTATAACATGTTGACTTGCGACAGCGGGGTGTGTCACATACTTAAGAACATACACGTCA
  CGAGCCACCCTCGCAAGAAATGGCCTGTATTTGAGAACAATCTGCTCATTAAGTGTCATATGCATCTCGG
  TGCTCGCCGCGGAACCTTCCAGCCCTACCAATGTAACTTTTCTCAGACTAAACTGCTGTTGGAAAATGAC
  GCATTCTCCAGAGTGAACCTGAATGGAATTTTCGATATGGACGTTTCTGTCTACAAGATCCTGCGCTACG
  ATGAAACCAAGTCCAGAGTCCGGGCTTGCGAGTGTGGCGGACGGCATACCAGGATGCAACCTGTCGCCCT
  GGACGTAACAGAAGAGCTCCGCCCCGACCATCTGGTCATGGCTTGCACAGGCACCGAATTCAGTAGCAGC
  GGAGAGGATACCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100149 representing YP_003038600
  Red=Cloning sites Green=Tags
  
  MEPGHPTKQGLHSGLCSHAPVEGLDEAAGTENLELLAFTASSVGSSSSTQTNIHVGGRNEGGHGREPEER
  PGPSVGRGAGLNEVSSLYPELSKVLTSMARGVKRERSDGGNTGLMTELTASLMNRKRPERITWHELQQEC
  RDEIGLMQDKYGLEQIKTHWLNPDEDWEEAIKKYAKIALRPDCKYRVTKTVNIKHACYISGNGAEVDIDT
  LDKSAFRCCMMGMRVGVMNMNSMIFINIKFNGEKFNGVLFMANSHMTLHGCSFFGFNNMCAEVWGAAKIR
  GCKFYGCWMGVVGRPKSEMSVKQCVFEKCYLGVCTEGNARVRHCSSLETGCFCLVKGTASIKHNVVKGCT
  DERMYNMLTCDSGVCHILKNIHVTSHPRKKWPVFENNLLIKCHMHLGARRGTFQPYQCNFSQTKLLLEND
  AFSRVNLNGIFDMDVSVYKILRYDETKSRVRACECGGRHTRMQPVALDVTEELRPDHLVMACTGTEFSSS
  GEDTD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012959
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012959.1, YP_003038600
• RefSeq ORF: 1485
• MW: 55.2 kDa