E1A (AC_000018) Virus Tagged ORF Clone

CAT#: VC100182

Myc-DDK-tagged ORF clone of viral ORF for E1A [Human adenovirus 7], codon optimized for human cell expression, AP_000534

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100182 represents NCBI reference of AP_000534 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGACATCTGAGATTCCTCCCACAGGAGATCATCTTTTCTGAGACTGGGATCGAGATCCTTGAATTTG
  TCGTTAACACGCTGATGGGCGACGATCCCGAGCCTCCAGTCCAGCCATTTGACCCGCCTACCCTTCATGA
  TCTGTATGATCTTGAGGTAGACGGACCCCAGGATCCCAATGAAGAGGCAGTGAACGGCTTTTTCACTGAC
  AGCATGCTCCTTGCTGCAGATGAAGGCCTCGATATTAACCCACCCCCCGAGACCCTCGTGACACCTGGAG
  TGGTCGTAGAGAGCGGGCGGGGGGGAAAGAAACTCCCTGATCTGGGCGCAGCAGAGATGGACCTTCGGTG
  CTATGAGGAGGGATTTCCCCCCTCTGATGACGAGGATGGCGAAACGGAACAGAGCATCCATACAGCTGTC
  AACGAAGGCGTTAAGGCCGCCAGCGACGTGTTTAAGCTCGATTGTCCTGAACTGCCAGGTCATGGGTGTA
  AGAGCTGCGAGTTTCACAGAAATAATACCGGGATGAAAGAACTCCTCTGCAGCCTGTGCTATATGCGAAT
  GCATTGCCATTTTATCTATTCCCCTGTCTCCGACGACGAGTCTCCCAGCCCGGACTCTACCACATCACCT
  CCGGAGATTCAAGCCCCCGCTCCAGCTAATGTTTGCAAGCCAATACCGGTCAAACCCAAGCCTGGAAAAC
  GCCCTGCTGTGGACAAGCTGGAGGACCTGTTGGAGGGTGGGGATGGGCCATTGGACTTGTCAACCCGCAA
  GCTCCCACGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100182 representing AP_000534
  Red=Cloning sites Green=Tags
  
  MRHLRFLPQEIIFSETGIEILEFVVNTLMGDDPEPPVQPFDPPTLHDLYDLEVDGPQDPNEEAVNGFFTD
  SMLLAADEGLDINPPPETLVTPGVVVESGRGGKKLPDLGAAEMDLRCYEEGFPPSDDEDGETEQSIHTAV
  NEGVKAASDVFKLDCPELPGHGCKSCEFHRNNTGMKELLCSLCYMRMHCHFIYSPVSDDESPSPDSTTSP
  PEIQAPAPANVCKPIPVKPKPGKRPAVDKLEDLLEGGDGPLDLSTRKLPRQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000534
• RefSeq ORF: 783
• MW: 28.5 kDa