E3 CR1-alpha0 (AC_000018) Virus Tagged ORF Clone

CAT#: VC100200

Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-alpha0 [Human adenovirus 7], codon optimized for human cell expression, AP_000556

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 CR1-alpha0
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100200 represents NCBI reference of AP_000556 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCCTTTGCAGTACTCTTTGTCTTGTCTCTGATTAAGACAGAGCTGAGGCCTTCCTATGGGTTGC
  CCCTGCTGCAGTCCGGCCTCTACAATACTAACCAAATTTTCCAGAAAACCCAGACTCTCCCTCCTCTGAT
  CCAAGATAGCAATTCAACGCTCCCTGCTCCTTCTACGACGAACCTCCCAGAGACAAATAAGCTTGGCAGC
  CACCTGCAGCACAGGCTTTCCCGGAGCTTGCTGTCTGCTAACACCACCACTCCCAAGACGGGTGGAGAGC
  TTAGGGGGCTGCCCACAGATGATCCTTGGGTAGTTGCTGGGTTCGTGACGCTTGGCGTCGTGGCAGGAGG
  ACTCGTGCTGATCCTGTGTTATCTCTATATCCCCTGCTGTGCTTATCTGGTCATCCTGCGGTGTTGGTTC
  AAGAAGTGGGGACCCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100200 representing AP_000556
  Red=Cloning sites Green=Tags
  
  MKAFAVLFVLSLIKTELRPSYGLPLLQSGLYNTNQIFQKTQTLPPLIQDSNSTLPAPSTTNLPETNKLGS
  HLQHRLSRSLLSANTTTPKTGGELRGLPTDDPWVVAGFVTLGVVAGGLVLILCYLYIPCCAYLVILRCWF
  KKWGPY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 438 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000556
• RefSeq ORF: 438
• MW: 16.1 kDa