III (AC_000018) Virus Tagged ORF Clone

CAT#: VC100186

Myc-DDK-tagged ORF clone of viral ORF for III [Human adenovirus 7], codon optimized for human cell expression, AP_000543

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: III
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100186 represents NCBI reference of AP_000543 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAAGACGCGCGGTCCTGGGAGGCGCGGTGGTCTACCCAGAAGGCCCTCCCCCATCCTATGAATCCG
  TGATGCAACAGCAGGCAGCCATGATACAGCCGCCTCTGGAAGTCCCATTCGTTCCCCCCCGGTACTTGGC
  ACCTACAGAAGGAAGGAACTCCATTAGATACAGCGAACTCTCTCCCCTGTATGACACAACAAAACTGTAC
  TTGGTTGATAACAAGAGTGCTGATATCGCCAGCCTTAATTACCAGAATGACCACTCTAATTTCCTGACCA
  CTGTCGTTCAGAATAATGACTTCACTCCCACTGAGGCCAGTACGCAGACCATAAATTTTGACGAGAGGTC
  CCGCTGGGGGGGACACCTGAAGACTATCATGCACACAAATATGCCCAATGTCAATGAGTATATGTTCTCT
  AATAAGTTCAAGGCGCGAGTTATGGTATCACGGAAGGCACCTGAAGGAGTTACTGTTAATGATACATACG
  ATCATAAAGAAGATATCCTGAAGTACGAGTGGTTCGAGTTCATCCTGCCCGAGGGCAACTTCTCCGCAAC
  AATGACGATAGATCTGATGAATAACGCGATCATCGATAACTATCTGGAGATTGGTCGGCAGAACGGCGTC
  CTGGAGAGCGATATTGGAGTCAAATTCGATACCCGGAATTTCAGACTGGGATGGGACCCAGAGACTAAGC
  TGATAATGCCGGGAGTTTATACCTATGAGGCCTTCCACCCCGACATTGTGCTTCTGCCGGGCTGCGGAGT
  TGATTTCACCGAATCACGCCTGTCAAACTTGCTCGGTATTCGGAAGCGCCATCCTTTTCAGGAGGGCTTC
  AAGATAATGTACGAAGATTTGGAAGGCGGCAACATCCCTGCCCTTCTGGACGTCACTGCCTATGAAGAGA
  GTAAGAAGGACACCACAACCGAGACCACAACACTGGCCGTTGCCGAGGAGACCAGCGAAGACGACAATAT
  AACTAGGGGTGATACATACATAACAGAGAAGCACAAAAGGGAGGCTGCTGCAGCCGAGGTCAAAAAAGAG
  CTGAAGATCCAGCCCCTTGAGAAAGATTCTAAGAGCCGCAGCTATAATGTGTTGGAGGATAAGATCAACA
  CTGCTTACAGGAGCTGGTACCTCTCCTATAATTACGGAAACCCCAAGAAGGGCATAAGATCTTGGACTCT
  CCTGACCACTAGCGACGTGACTTGTGGCGCGGAGCAGGTGTACTGGTCACTCCCAGACATGATGCAGGAC
  CCTGTTACTTTCAGATCCACCCGGCAGGTGAATAATTATCCAGTCGTTGGCGCCGAGCTGATGCCAGTCT
  TTAGCAAGAGTTTTTATAATGAGCAGGCTGTGTACTCTCAGCAGTTGCGGCAGGCTACTAGTCTCACTCA
  CGTGTTTAATAGGTTTCCAGAGAACCAGATCCTCATTAGGCCACCCGCACCAACCATTACCACTGTCAGC
  GAGAACGTCCCAGCATTGACCGACCACGGAACCCTCCCCCTTCGGTCATCAATTCGAGGAGTGCAAAGAG
  TTACAGTGACCGATGCTAGGCGGCGGACTTGTCCTTATGTATACAAAGCACTGGGCATAGTGGCCCCCCG
  GGTGCTGTCCAGCAGGACTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100186 representing AP_000543
  Red=Cloning sites Green=Tags
  
  MRRRAVLGGAVVYPEGPPPSYESVMQQQAAMIQPPLEVPFVPPRYLAPTEGRNSIRYSELSPLYDTTKLY
  LVDNKSADIASLNYQNDHSNFLTTVVQNNDFTPTEASTQTINFDERSRWGGHLKTIMHTNMPNVNEYMFS
  NKFKARVMVSRKAPEGVTVNDTYDHKEDILKYEWFEFILPEGNFSATMTIDLMNNAIIDNYLEIGRQNGV
  LESDIGVKFDTRNFRLGWDPETKLIMPGVYTYEAFHPDIVLLPGCGVDFTESRLSNLLGIRKRHPFQEGF
  KIMYEDLEGGNIPALLDVTAYEESKKDTTTETTTLAVAEETSEDDNITRGDTYITEKHKREAAAAEVKKE
  LKIQPLEKDSKSRSYNVLEDKINTAYRSWYLSYNYGNPKKGIRSWTLLTTSDVTCGAEQVYWSLPDMMQD
  PVTFRSTRQVNNYPVVGAELMPVFSKSFYNEQAVYSQQLRQATSLTHVFNRFPENQILIRPPAPTITTVS
  ENVPALTDHGTLPLRSSIRGVQRVTVTDARRRTCPYVYKALGIVAPRVLSSRTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 1632 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000543
• RefSeq ORF: 1632
• MW: 61.9 kDa