pVII (AC_000018) Virus Tagged ORF Clone

CAT#: VC100189

Myc-DDK-tagged ORF clone of viral ORF for pVII [Human adenovirus 7], codon optimized for human cell expression, AP_000544

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100189 represents NCBI reference of AP_000544 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTATATTGATATCCCCAAGCAACAATACCGGATGGGGCCTGTACGCCCCTACTAAGATGTACGGTG
  GAGCTAGAAAACGCAGCACTCAGCATCCCGTCCGCGTGAGAGGCCACTTTCGGGCACCATGGGGTGCCCT
  GAAAGGGAGAACCCGCACCCGGACGACGGTTGATGACGTGATCGACCAGGTCGTGGCGGACGCCAGAAAT
  TACACCCCTACAGCCCCCACGTCTACTGTTGACGCTGTAATCGACTCTGTGGTCGCTGATGCCCGAGCCT
  ACGCCCGCAGGAAATCCAGACGCCGGCGCATCGCAAGGCGGCACCGAGCTACTCCTGCCATGCGGGCCGC
  CAAGGCAATCCTCCGGAGAGCAAAACGGGTGGGGAGACGGGCAATGCTTCGAGCTGCACGGAGGGCCGCC
  TCCGGGGCCTCTTCTGGTAGATCTCGAAGGAGGGCTGCTACAGCTGCTGCTGCAGCCATTGCCAACATGG
  CTCAGCCACGGAGAGGTAACGTGTATTGGGTGCGAGATGCCACGACAGGGCAAAGAGTGCCCGTGAGAAC
  TAGGCCACCCAGGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100189 representing AP_000544
  Red=Cloning sites Green=Tags
  
  MSILISPSNNTGWGLYAPTKMYGGARKRSTQHPVRVRGHFRAPWGALKGRTRTRTTVDDVIDQVVADARN
  YTPTAPTSTVDAVIDSVVADARAYARRKSRRRRIARRHRATPAMRAAKAILRRAKRVGRRAMLRAARRAA
  SGASSGRSRRRAATAAAAAIANMAQPRRGNVYWVRDATTGQRVPVRTRPPRT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 576 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000544
• RefSeq ORF: 576
• MW: 21.2 kDa