pTP (AC_000018) Virus Tagged ORF Clone

CAT#: VC100215

Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus 7], codon optimized for human cell expression, AP_000540

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pTP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100215 represents NCBI reference of AP_000540 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTGTCTATCAACGACTGTGCGAGGCTGACCGGTCAAACCGTGCCTACAATGAATTACTTCATGC
  CTCTGCGAAATATATGGAATCGGGTAAGGGAGTTTCCGAGAGCTAGCACTACAGCCTCAGGTATTACATG
  GATGTCCCGGTATATTTACGGCTATCATAGATTGATGCTCGAAGATCTTGCGCCGGGAGCTCCCGCCACA
  GAGCGCTGGCCACTGTACCGCCAGCCACCGCCCCACTTCCTTATTGGCTACCAGTACTTGGTGAGGACAT
  GCAATGACTACATTTTCGATACCCGAGCTTATTCACGGCTGAAGTACACAGAACTCGTGCGCCCCGGGCA
  CCAGACCGTGAACTGGAGCGTCATGGCCAATTGTAGTTATACAATCAATACAGGGGCTTATCATAGATTT
  GTTGACTTCGATGATTTTCAGGCTACACTCACCCAGGTGCAACAAGCTATCTTGGCAGAGAGAGTTGTAG
  CCGATCTCGCACTGGTGCAGCCGATGAGAGGCTTCGGAATTACCCGGATGCATGGGCGGGCGGGCGAGGA
  GGAGGTCCCCGTGGAGCGCCTGATGCAAGATTACTACAAAGACCTGGCACGGTGTCAGGACAACGCATGG
  GGAATGGCTCACAGACTCCGAATTCAGCAGGCAGGTCCGAAGGACCTGGTGCTTCTCGCCACAATCCGGA
  GACTCAAGACTGCTTATTTTAATTTCATCACTTCTTCTATTGTTTCCAGTTCTCAGGAGGAGGAGGGTAA
  AGAGAGGGAGAATCCTGATCGGGCAAGCAGTAGACCAAGGCCGCAGGAAACTGTGTTGTCCCTCCCTTGC
  GATTGCGATTGGTTGGACGCCTTCGTGGAGAGATTTTCAGATCCTGTAGACTTGGAAACCATCAGGTCAC
  TGCGCGGGGTGCCAACTGGTCAGCTCATCAAATGCATTATCAGCGCCGTATCCCTGCCTAATGGCGAGCC
  ACCTAGTCACCATTTTCGCGAGATGAGGGGAGGGGTTTTCACGTTGCGGCCCCGGGAAAACGGGAGAGCG
  GTCACCGAGACTATGAGGAGAAGGCGGGGAGAAGTGATCGAGAGGTTCATCGACAGATTGCCAGTGAGAC
  GCCGGAGACGCAGGGTTCCCCCCCCACCAGCTGCACCCCCAGAAGAGGAGGAGATGCTTGTCGAGGAAGA
  GGAAATCGAAGAAGAGATACCAGGGGACTTCGAACGGGAAGTAAGGACAACCATCGCTGAACTCATTAGG
  CTCCTGGAGGAGGAGTTGACTGTGTCAGCCCGCAATTCTCAGTTCTTCAACTTCGCTGTAGATTTCTACG
  AAGCAATGGAGCGGCTCGAGGCGCTGGGAGACGTATCCGAGATGCCCCTTCGGAGATGGATCATGTATTT
  TTTTGTTACAGAGCACATCGCTACCACCTTGAACTACCTGTTTCAACGCCTCTGTAATTACGCTGTCTTT
  ACTCGCCACGTGGAGCTGAACCTGGCCCAGGTTGTAATGAGGGCTCGCGATCCTGTTGGCGCTGTGGTGT
  ACTCTCGAGTTTGGAACGAGGCCGGAATGAATGCTTTTTCCCAGTTGATAGGGCGCATTTCAAATGACCT
  GGCGGCTACTGTTGAGCGGGCCGGCAGGGGTGACCTCCAGGAGGAAGAGATCCAGCAATTCATGGCAGAG
  ATTGCGTACCAGGATAACTCTGGAGATGTTCAGGAGATTCTGCGACAGGCTGCCGTTAACGATACTGAGA
  TCGATTCAGTGGAGCTGTCTTTTCGATTCAAACTCACCGGACCAGTCGCTTTTACCCAACGGAGGCAGAT
  TCAGGACGTTAACAGGCGAGTAGTTGCTCACGCTTCCCTGCTGAGGGCTCAGTACCAGAATCTTCCCGCC
  CGCGGGGCTGATGTTCCCCTTCCTGCCATGCCTCCAGGTCCTGAACCTCCCCTGCCTCCGGGGGCTAGAC
  CCAGGCACCGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100215 representing AP_000540
  Red=Cloning sites Green=Tags
  
  MALSINDCARLTGQTVPTMNYFMPLRNIWNRVREFPRASTTASGITWMSRYIYGYHRLMLEDLAPGAPAT
  ERWPLYRQPPPHFLIGYQYLVRTCNDYIFDTRAYSRLKYTELVRPGHQTVNWSVMANCSYTINTGAYHRF
  VDFDDFQATLTQVQQAILAERVVADLALVQPMRGFGITRMHGRAGEEEVPVERLMQDYYKDLARCQDNAW
  GMAHRLRIQQAGPKDLVLLATIRRLKTAYFNFITSSIVSSSQEEEGKERENPDRASSRPRPQETVLSLPC
  DCDWLDAFVERFSDPVDLETIRSLRGVPTGQLIKCIISAVSLPNGEPPSHHFREMRGGVFTLRPRENGRA
  VTETMRRRRGEVIERFIDRLPVRRRRRRVPPPPAAPPEEEEMLVEEEEIEEEIPGDFEREVRTTIAELIR
  LLEEELTVSARNSQFFNFAVDFYEAMERLEALGDVSEMPLRRWIMYFFVTEHIATTLNYLFQRLCNYAVF
  TRHVELNLAQVVMRARDPVGAVVYSRVWNEAGMNAFSQLIGRISNDLAATVERAGRGDLQEEEIQQFMAE
  IAYQDNSGDVQEILRQAAVNDTEIDSVELSFRFKLTGPVAFTQRRQIQDVNRRVVAHASLLRAQYQNLPA
  RGADVPLPAMPPGPEPPLPPGARPRHRS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 1974 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000540
• RefSeq ORF: 1974
• MW: 75.6 kDa