E1B 19K (AC_000018) Virus Tagged ORF Clone

CAT#: VC100183

Myc-DDK-tagged ORF clone of viral ORF for E1B 19K [Human adenovirus 7], codon optimized for human cell expression, AP_000535

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B 19K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100183 represents NCBI reference of AP_000535 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTTTGGGCCATCCTCGAAGACTTGAGGCAAACAAGACTCCTGCTCGAGAACGCAAGCGACGGGG
  TGAGTGGGCTGTGGAGGTTCTGGTTTGGTGGGGATCTGGCGAGACTGGTGTTCCGGATTAAACAGGACTA
  TTCCGTCGAGTTCGAGAAGCTCCTGGATGATTCACCCGGCCTGTTCGAAGCCCTGAACTTGGGGCATCAG
  GCCCATTTCAAAGAGAAAGTCCTGAGTGTGCTGGATTTTTCTACACCCGGAAGGACCGCAGCCGCGGTGG
  CTTTCCTTACTTTCATCTTGGATAAGTGGATTCGCCAGACTCACTTCAGTAAGGGCTACGTACTTGACTT
  CATTGCTGCAGCGCTGTGGAGAACGTGGAAAGCCAGAAGAATGAGGACGATCCTCGACTACTGGCCGGTG
  CAGCCGCTTGGGGTGGCCGGCATTCTGAGACATCCCCCAACGATGCCTGCGGTCTTGCAAGAGGAGCAAC
  AGGAAGACAACCCACGGGCCGGACTCGATCCACCTGTCGAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100183 representing AP_000535
  Red=Cloning sites Green=Tags
  
  MEVWAILEDLRQTRLLLENASDGVSGLWRFWFGGDLARLVFRIKQDYSVEFEKLLDDSPGLFEALNLGHQ
  AHFKEKVLSVLDFSTPGRTAAAVAFLTFILDKWIRQTHFSKGYVLDFIAAALWRTWKARRMRTILDYWPV
  QPLGVAGILRHPPTMPAVLQEEQQEDNPRAGLDPPVEE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 534 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000535
• RefSeq ORF: 534
• MW: 20.4 kDa