E4 ORF4 (AC_000018) Virus Tagged ORF Clone

CAT#: VC100209

Myc-DDK-tagged ORF clone of viral ORF for E4 ORF4 [Human adenovirus 7], codon optimized for human cell expression, AP_000567

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4 ORF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100209 represents NCBI reference of AP_000567 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATACTGCCGTCCTTGCCCTCTCCCCTCCTCCTGGAAACACAGTCTAGTTGCATAGCTTGGCTTGGCT
  TTGCATACGCAACCGTCGACGACTTCCTCAGAACTATTAAATACGACGGGGTCCTTATAACGACTGAAGC
  TAGCATCCTCCTGACTAATCTGCGCGTGTGGCTGTACTTGAACTACCAGACGGAGCATACGAAGAGGCAG
  GACCGACGCCGGAGGAGGGTGTGTTGCGCCAGAGCAGCCTTCTGTTACACAAAATATGAGAACGTCAGAA
  AGCAGTTGCATTACGACAAGGTTGCTAGAACTCTCGATATCATGCGCCAGACATCTCCCTTGCACCAATC
  CCCAGTTACTACCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100209 representing AP_000567
  Red=Cloning sites Green=Tags
  
  MILPSLPSPLLLETQSSCIAWLGFAYATVDDFLRTIKYDGVLITTEASILLTNLRVWLYLNYQTEHTKRQ
  DRRRRRVCCARAAFCYTKYENVRKQLHYDKVARTLDIMRQTSPLHQSPVTTM
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 366 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000567
• RefSeq ORF: 366
• MW: 14.3 kDa