pVI (AC_000018) Virus Tagged ORF Clone

CAT#: VC100191

Myc-DDK-tagged ORF clone of viral ORF for pVI [Human adenovirus 7], codon optimized for human cell expression, AP_000547

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100191 represents NCBI reference of AP_000547 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGATATCAACTTCTCAAGCCTTGCACCCCGGCATGGGACTAGGCCTTATATGGGAACTTGGAGTG
  ACATTGGCACCTCACAGCTTAACGGTGGGGCCTTTAACTGGTCCTCAATCTGGTCAGGTCTCAAGAACTT
  TGGATCAACTGTCAAGACATACGGAAACAAAGCGTGGAACAGCTCCACAGGACAGGCCCTGCGAAACAAA
  CTGAAGGAGCAGAACTTTCAGCAGAAAGTTGTCGACGGCATCGCTTCCGGCATTAACGGGGTGGTAGACC
  TGGCCAACCAAGCCGTTCAGAAACAGATCAACAGTAGGCTTGACCCTCCACCAGCTACCCCCGGTGAGAT
  GGAAGTGGAGGAGGAGCTGCCTCCTTTGGAGAAACGGGGGGACAAGCGCCCCCGACCCGAACTGGAGCAC
  ACCCTGGTTACGCGCGCCGACGAACCACCATCCTATGAAGAGGCAGTTAAACTGGGCATGCCCACTACAC
  GACCAGTAGCTCACATGGCAACCGGTGTCATGAAGCCTAGTGAGCTGCATAGGCCTGCCACATTGGATCT
  CCCCCCCCCTCCAGCCTCCGCAGCCCCCGTTCCAAAGCCTGTGGCAACACGAAAGCCCACAGCCGTGCAG
  CCTGTAGCTGTTGCCCGCCCCAGACCTGGGGGCACACCACGCCCCAAGGCTAACTGGCAGAGTACCCTCA
  ACTCCATTGTCGGACTTGGCGTTCAATCAGTAAAGCGGAGGCGCTGTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100191 representing AP_000547
  Red=Cloning sites Green=Tags
  
  MEDINFSSLAPRHGTRPYMGTWSDIGTSQLNGGAFNWSSIWSGLKNFGSTVKTYGNKAWNSSTGQALRNK
  LKEQNFQQKVVDGIASGINGVVDLANQAVQKQINSRLDPPPATPGEMEVEEELPPLEKRGDKRPRPELEH
  TLVTRADEPPSYEEAVKLGMPTTRPVAHMATGVMKPSELHRPATLDLPPPPASAAPVPKPVATRKPTAVQ
  PVAVARPRPGGTPRPKANWQSTLNSIVGLGVQSVKRRRCY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 750 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000547
• RefSeq ORF: 750
• MW: 27.2 kDa