DBP (AC_000018) Virus Tagged ORF Clone

CAT#: VC100194

Myc-DDK-tagged ORF clone of viral ORF for DBP [Human adenovirus 7], codon optimized for human cell expression, AP_000550

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100194 represents NCBI reference of AP_000550 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGTCGCGGGGGTAATCAGAGTTCCGACAGACAGCGGGAGCACACTCCTGAAAGAGGTATGGGAT
  CTGCTTCTCACCCTCTGCCGCGCCCGGATAGAAGCCCCTCTCAGAGCCCGCCACCACTGCCACCTAAACG
  AAATACTTACCGCCGCGTGGGTTCCGACTCTTCCATCGAAAGTCAGGTTGTGCTGGTGTCTGAAACATCC
  AGGAGCAGTCTCAGCCCGGAAAGATCAAATTCACCTCCACCTATCCCCCCCAAGAAGAAGCCCCGCAAGA
  CAAAGCACGTGCCACTGCAGGACATTGGGCACGATAGTGAGGAGGAACGGGAACAGGCCCAGCTGGTGGC
  CGTGGGATTCAGCTATCCCCCAGTACGCATAATCGAAAAGGACGGTAAGAGATCTGTTGAGAAAATTGAC
  AAAAACGACCCCATTGCTAAAGGAGCTACTAGCATAGCTGTGAGGAATCCTCTGTCCCTCCCCTTGGTTT
  CTGCATGGGAAAAAGGAATGGAGGTCATGGCTGTCTTGATGGAAAGATATCGCTTGGACAATGACCTCAG
  GACGAGTTTTAAACTGATGCCTGAGCAACACGAACAGTATAAACGCATATGCCACCAATATGTCAATGAG
  GAACACCGGGGAATTCCACTCACCTTCAGCTCAATGAAGACCCTGACCGCCATGATGGGCCGATTCATGC
  AGGGTCTGGTGCATTCTTATTCCGAGATAGCTCACAACAGCTGGGAATGTACCGGCTGTGCCCTGTGGGC
  TCACGGATGTACCGATTACGAGGGTAAAGTGAAATGCCTCCACGGCACCATAATGATCCAGAAGGATCAC
  ATTATTGAGATGGACGTGGCCAGCGAGAACGGTCAGCGCGCTATGAAAGAGAACCCAGACAGGGCCAAGA
  TCACCCAGAACCGATGGGGCCGAAATGTGGTGCAGCTGGCTAACAACGACGCCCGATGTTGTGTGAACGA
  CGCCAATTGTGCCACCAACCAGTTCTCTTCCAAGAGCTGCGGTATGTTTTACACGGAGGGCTCAAAGGCT
  CAAGAGGCGTTTAAGCAGTATGAAGCCTTTATGAAGGCAGTGTATCCAGGCATTACTCCAGACCAAGCTC
  GGATGATGCTGATCCCTATCCACTGCGACTGTAATCATAAGCCTGGCTGCGCCCCCGTCATGGGTCGACA
  GACATGCAAAATGACACCCTTCGGGATGGCTAACGCCGAGGACCTGGACGTCACCACAATCTCTGATCCT
  ACAGTACTTGCCAGTGTCCGCCACCCTGCCCTCATGGTGTTTCAGTGTTGTAATCCCGTCTATCGCAATT
  CTAGGGTGCAAAACGCCGGGCCAAATTGCGACTTTAAGATCAGTGCACCCGACCTTCTGGGCGCGTTGCA
  GTTGACCAGGAAACTGTGGCAGGACACATTTCCCGAAATTCCAGTCCCTAAACTTGTAATTCCCGAATTT
  AAGTGGCAAAACCGCCTCCAGTTCCGGAACGTCTCCTTGCCAGCCGGCCATTTCGATAGTCGACAGAACC
  CCTTCGATTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100194 representing AP_000550
  Red=Cloning sites Green=Tags
  
  MASRGGNQSSDRQREHTPERGMGSASHPLPRPDRSPSQSPPPLPPKRNTYRRVGSDSSIESQVVLVSETS
  RSSLSPERSNSPPPIPPKKKPRKTKHVPLQDIGHDSEEEREQAQLVAVGFSYPPVRIIEKDGKRSVEKID
  KNDPIAKGATSIAVRNPLSLPLVSAWEKGMEVMAVLMERYRLDNDLRTSFKLMPEQHEQYKRICHQYVNE
  EHRGIPLTFSSMKTLTAMMGRFMQGLVHSYSEIAHNSWECTGCALWAHGCTDYEGKVKCLHGTIMIQKDH
  IIEMDVASENGQRAMKENPDRAKITQNRWGRNVVQLANNDARCCVNDANCATNQFSSKSCGMFYTEGSKA
  QEAFKQYEAFMKAVYPGITPDQARMMLIPIHCDCNHKPGCAPVMGRQTCKMTPFGMANAEDLDVTTISDP
  TVLASVRHPALMVFQCCNPVYRNSRVQNAGPNCDFKISAPDLLGALQLTRKLWQDTFPEIPVPKLVIPEF
  KWQNRLQFRNVSLPAGHFDSRQNPFDF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000550
• RefSeq ORF: 1551
• MW: 58.1 kDa