33K (AC_000018) Virus Tagged ORF Clone

CAT#: VC100197

Myc-DDK-tagged ORF clone of viral ORF for 33K [Human adenovirus 7], codon optimized for human cell expression, AP_000552

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 33K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100197 represents NCBI reference of AP_000552 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCACCGACGCGGTTCCAAGAAGCTCAAGGTGCAGCTGACCCCCCCAGAAGACATGGAAGAGGATT
  GGGACAGCCAGGCTGAGGAGGAGATGGAGGATTGGGATAGTCAGGCTGAAGAGGTCGATAGTCTGGAGGA
  GGACAGCCTTGAAGAGGAAGATGAGGAGGCTGAGGAAGTGGAGGAAGCAACAGCAGCCAAACAGCTCTCT
  TCAGCTGCTGAAACCTCAAAATCACCGGACAGCAGCACAGCCACCATTTCCGCACCCGGTCGGGGCGTTC
  AGCGACGACCTAATAGTAGATGGGATGAGACTGGCCGCTTCCCGAACCCTACAACAGCCTCTAAGACCGC
  ACCTACCGCCGTTAGTAAGTCCAGGCAACCCCGACAGAAAAAAACAGCCGCAACCACAGTAACTAGGAAG
  CCTGCCGTGCGGAAGAGCACTACTAGTGCCGCCGGGGGCGGTCTGAGAATCACTGCGAATGAGCCAGCAC
  AAACTCGCGAGCTGAGAAACAGGATCTTTCCCACACTTTATGCCATCTTTCAGCAGTCACGGGGGCAGGA
  GCAGGAGCTCAAAGTGAAGAATAGAAGCCTGAGATCCTTGACCAGGTCATGCCTGTACCACAAAAGTGAA
  GATCAGCTGCAACGCACCCTGGAAGACGCAGAGGCTCTGTTCAATAAATATTGTGCCCTGACTCTGAAAG
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100197 representing AP_000552
  Red=Cloning sites Green=Tags
  
  MSHRRGSKKLKVQLTPPEDMEEDWDSQAEEEMEDWDSQAEEVDSLEEDSLEEEDEEAEEVEEATAAKQLS
  SAAETSKSPDSSTATISAPGRGVQRRPNSRWDETGRFPNPTTASKTAPTAVSKSRQPRQKKTAATTVTRK
  PAVRKSTTSAAGGGLRITANEPAQTRELRNRIFPTLYAIFQQSRGQEQELKVKNRSLRSLTRSCLYHKSE
  DQLQRTLEDAEALFNKYCALTLKE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 702 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000552
• RefSeq ORF: 702
• MW: 26.2 kDa