pIIIa (AC_000018) Virus Tagged ORF Clone

CAT#: VC100188

Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus 7], codon optimized for human cell expression, AP_000542

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pIIIa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100188 represents NCBI reference of AP_000542 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGCAGCAGGCGCCTGATCCCGCCATGCGCGCAGCCCTGCAGTCTCAGCCAAGCGGCATCAACT
  CCAGTGACGACTGGACACAAGCGATGCAGCGGATTATGGCATTGACTACCCGGAATCCCGAGGCTTTTCG
  GCAGCAGCCGCAGGCTAATAGACTCTCCGCCATCCTGGAAGCAGTTGTCCCCAGCCGATCTAACCCAACT
  CACGAGAAGGTTCTCGCCATCGTAAACGCCCTCGTGGAGAACAAGGCAATCAGACCGGACGAAGCAGGGT
  TGGTGTATAACGCCCTCCTGGAGCGCGTGGCTCGATACAATTCCTCTAATGTCCAGACAAACTTGGACCG
  GATGGTCACCGATGTGAGGGAGGCTGTCAGCCAGCGAGAGAGATTTCAGAGGGGGGCTAATTTGGGGAGC
  CTGGTGGCTTTGAACGCATTTTTGAGCACACAGCCTGCCAATGTGCCGCGAGGACAGCAGGACTATACCA
  ATTTTCTTAGCGCTCTTAGGCTTATGGTCGCGGAAGTCCCTCAGTCAGAAGTGTATCAGAGTGGCCCTGA
  TTATTTCTTTCAGACTTCACGGCAGGGCCTGCAAACTGTCAATCTTACACAGGCCTTTAAGAATCTGAAG
  GGTTTGTGGGGCGTGCATGCGCCAGTTGGGGACCGCGCTACCGTGAGTTCTCTGCTCACCCCCAACTCAA
  GACTCCTGCTCCTGCTCGTTAGCCCTTTCACTGACTCTGGTTCTATCGATCGCAACTCCTACCTGGGCTA
  CCTGCTGAACCTGTATAGGGAGGCTATTGGTCAATCTCAGGTCGATGAGCAAACATACCAGGAGATAACA
  CAGGTTTCCCGAGCACTGGGACAGGAAGATACCGGGTCTCTTGAAGCAACACTCAATTTTCTGCTGACGA
  ACAGGTCCCAAAAAATTCCGCCTCAGTATGCCTTGACCGCCGAAGAGGAGCGCATCTTGCGCTACGTGCA
  GCAGTCTGTCGGTCTTTTTCTTATGCAAGAGGGCGCAACTCCCACTGCTGCGCTTGACATGACAGCTCGC
  AACATGGAACCATCTATGTACGCCAGCAATAGGCCTTTCATCAATAAGCTGCTCGACTATTTGCACCGGG
  CTGCCGCAATGAACTCCGACTACTTTACTAATGCCATTTTGAACCCGCATTGGCTCCCCCCCCCGGGCTT
  CTACACTGGCGAGTACGATATGCCAGATCCCAATGACGGCTTTCTGTGGGACGACGTCGATTCAAGTATT
  TTTTCTCCACCACCAGGTTACACCGTTTGGAAGAAAGAAGGAGGAGATCGACGCCACAGTAGCGTGAGCC
  TTTCTGGCACCGCAGGGGCAGCCGCAACTGTCCCAGAAGCCGCTTCACCATTCCCAAGCCTTCCGTTTTC
  TCTGAACAGTGTCCGCTCCTCCGAGCTGGGGAGAATTACACGGCCCAGACTTATGGGAGAAGACGAGTAC
  TTGAACGATTCTCTTCTCAGACCCGAAAGAGAAAAGAACTTCCCGAATAATGGAATCGAATCCCTGGTGG
  ACAAAATGAGTCGATGGAAGACTTACGCACAGGATCATCGGGATGAGCCTAGAATCCTGGGGGCCACCTC
  CCGCCCTACGCGGCGCCGCCAGAGGCATGATAGGCAGATGGGTTTGGTGTGGGACGATGAAGATTCCGCT
  GATGACAGCTCCGTACTCGATTTGGGCGGACGCGGTGGAGGAAACCCGTTCGCACATCTCCGGCCTCGAT
  TTGGCCGGATGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100188 representing AP_000542
  Red=Cloning sites Green=Tags
  
  MEQQQAPDPAMRAALQSQPSGINSSDDWTQAMQRIMALTTRNPEAFRQQPQANRLSAILEAVVPSRSNPT
  HEKVLAIVNALVENKAIRPDEAGLVYNALLERVARYNSSNVQTNLDRMVTDVREAVSQRERFQRGANLGS
  LVALNAFLSTQPANVPRGQQDYTNFLSALRLMVAEVPQSEVYQSGPDYFFQTSRQGLQTVNLTQAFKNLK
  GLWGVHAPVGDRATVSSLLTPNSRLLLLLVSPFTDSGSIDRNSYLGYLLNLYREAIGQSQVDEQTYQEIT
  QVSRALGQEDTGSLEATLNFLLTNRSQKIPPQYALTAEEERILRYVQQSVGLFLMQEGATPTAALDMTAR
  NMEPSMYASNRPFINKLLDYLHRAAAMNSDYFTNAILNPHWLPPPGFYTGEYDMPDPNDGFLWDDVDSSI
  FSPPPGYTVWKKEGGDRRHSSVSLSGTAGAAATVPEAASPFPSLPFSLNSVRSSELGRITRPRLMGEDEY
  LNDSLLRPEREKNFPNNGIESLVDKMSRWKTYAQDHRDEPRILGATSRPTRRRQRHDRQMGLVWDDEDSA
  DDSSVLDLGGRGGGNPFAHLRPRFGRML
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 1764 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000542
• RefSeq ORF: 1764
• MW: 65.7 kDa