E4 34K (AC_000018) Virus Tagged ORF Clone

CAT#: VC100208

Myc-DDK-tagged ORF clone of viral ORF for E4 34K [Human adenovirus 7], codon optimized for human cell expression, AP_000566

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4 34K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100208 represents NCBI reference of AP_000566 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGGTCCAATTCTATAATGACCAGACTTAGGGCTCGGTCCACATCATGTGCTAGACACCATCCCT
  ACACCCGCGCCCAGCTTCCAAGATGCGAAGAAAATGAAACCAGAGCATCTATGACAGAAGATCATCCGCT
  GCTGCCAGACTGTGATACTATGACTATGCACTCAGTATCTTGTGTGCGCGGCCTCCCCTGCAGCGCCAGT
  TTCACAGTCCTGCAGGAGTTTCCTATTCCCTGGGATATGTTTCTGAACCCGGAGGAACTCCGCATCATGC
  GCAGGTGTATGCACCTGTGCCTGTGCTGTGCCACAATTGATATCTTCTACAGTCAGGTGATCCACGGGAG
  AGAAAATTGGGTACTGCACTGCCACTGCAACCGCCAGGGATCTCTTCAGTGTATGGCAGGCGGTGCCGTT
  CTGGCAGTGTGGTTTCGAAAAGTCATCCTGGGGTGTATGATTAACCAGCGGTGCCCTTGGTACAGACAAA
  TAGTAAATATGCACATGCCTAAGGAAATAATGTATGTAGGGTCAGTATTCCTGAGAGAGAGGCATCTGAT
  CTACATCAAAATCTGGTATGATGGACACGCCGGCGCGATAATTAGCGATATGAGTTTTGGATGGTCTGCT
  TTCAATTACGGCTTGTTGAACAATATCGTGATCATGTGCTGTACCTATTGTTCAAATCTGTCCGAGATTC
  GGATGCGGTGCTGTGCCCACAGAACCCGGAAGTTGATGCTTCGAGCTATCAAAATCATGTTGCAGGATAC
  CGTGGACCCAGATCCAATCAACTCTTCACGGACGGAGCGCAGAAGGCAGCGCCTGCTCGTGGGCTTGATG
  CGACACAACCGCCCTATTCCTTTTTCAGATTACGATTCTCACCGGAGCTCTTCTCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100208 representing AP_000566
  Red=Cloning sites Green=Tags
  
  MSGSNSIMTRLRARSTSCARHHPYTRAQLPRCEENETRASMTEDHPLLPDCDTMTMHSVSCVRGLPCSAS
  FTVLQEFPIPWDMFLNPEELRIMRRCMHLCLCCATIDIFYSQVIHGRENWVLHCHCNRQGSLQCMAGGAV
  LAVWFRKVILGCMINQRCPWYRQIVNMHMPKEIMYVGSVFLRERHLIYIKIWYDGHAGAIISDMSFGWSA
  FNYGLLNNIVIMCCTYCSNLSEIRMRCCAHRTRKLMLRAIKIMLQDTVDPDPINSSRTERRRQRLLVGLM
  RHNRPIPFSDYDSHRSSSR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000566
• RefSeq ORF: 897
• MW: 34.8 kDa