E3 RID-beta (AC_000018) Virus Tagged ORF Clone

CAT#: VC100205

Myc-DDK-tagged ORF clone of viral ORF for E3 RID-beta [Human adenovirus 7], codon optimized for human cell expression, AP_000561

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 RID-beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100205 represents NCBI reference of AP_000561 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCCATGCTGCCCGTCATACTCATCCTTCTGCTTCCTTGTATCGCACTGGCGTCTACCGCAACCC
  GGGCCACTCCAGAACAGCTGCGAAAATGCAAGTTCCAACAGCCTTGGTCTTTCCTCGATTGTTACCATGA
  GAAATCCGACTTTCCAACCTATTGGATCGTAATTGTTGGCATCATCAACATCTTGTCATGCACGTTTTTT
  TCCATTACCATCTATCCCACGTTCAACTTCGGATGGAACTCTCCCAACGCTCTTGGGTACCCCCAGGAGC
  CCGACGAGCACATTCCCTTGCAGCACATCCAGCAGCCCCTTGCCTTGGTGGAGTACGAGAACGAACCACA
  ACCCTCTTTGCCTCCAGCTATCTCCTATTTTAATCTCACTGGGGGGGATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100205 representing AP_000561
  Red=Cloning sites Green=Tags
  
  MQAMLPVILILLLPCIALASTATRATPEQLRKCKFQQPWSFLDCYHEKSDFPTYWIVIVGIINILSCTFF
  SITIYPTFNFGWNSPNALGYPQEPDEHIPLQHIQQPLALVEYENEPQPSLPPAISYFNLTGGDD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 402 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000561
• RefSeq ORF: 402
• MW: 15.2 kDa