IVa2 (AC_000018) Virus Tagged ORF Clone

CAT#: VC100213

Myc-DDK-tagged ORF clone of viral ORF for IVa2 [Human adenovirus 7], codon optimized for human cell expression, AP_000538

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IVa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100213 represents NCBI reference of AP_000538 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACCAAGGGACGGAGGAGCGCCGCTGTGCTCGATCAGCAGGACGAGTCCGAAGCCCACCCGAGGA
  AAAGACCTACAAGAAGAGCACCTATCCATCGCGATGGTAATAATCCCGACAGCGACGCAGCCACCATGGA
  AGGCAGTGACCCAGGCTCCGCAGGTAGGCCATCTAGCGACTCCTTGTTGCAAGAACCTTCCCAACCAGCC
  AAGAGAGGGGGACTCTTGGATCGAGACGCTATCGAGCACATTACAGAACTTTGGGACAGACTCGAGCTGC
  TCCAACAAACTCTGTCTAAGATGCCAATGGCTGATGGACTCAAACCACTGAAGAACTTCAGCTCTCTCCA
  GGAGCTGCTGAGTTTGGGCGGCGAGAGGCTGCTCACCGACCTTGTTCGCGAAAACATACACGTCCGAGAG
  ATGATGAATGAGGTCGCTCCATTGCTGCGCGAAGATGGCTCTTGTAGGTCTCTGAACTATCATCTGCAGC
  CAGTAATCGGCGTCATTTACGGGCCGACAGGATGCGGTAAATCTCAACTTCTCAGGAACCTGCTTTCCTC
  TCAGCTGATTACACCAGCACCTGAAACCGTCTTCTTTATTGCGCCCCAGGTTGACATGATACCTCCTTCA
  GAACTGAAGGCATGGGAGATGCAGATCTGTGAGGGGAATTATGCTCCCGGTCCCGAGGGAACTTTCATTC
  CTCAATCAGGAACGCTTCGGCCAAAGTTCATTAAAATGGCCTACGATGACCTGACTCAGGAGCACAATTA
  CGATGTGAGCGATCCCAGGAACGTGTTCGCCAGAGCCGCAGCACACGGTCCTATTGCAATCATTATGGAT
  GAGTGCATGGAAAACCTGGGCGGACATAAGGGAGTCAGCAAGTTCTTCCACGCCTTTCCCTCAAAGCTGC
  ATGATAAGTTCCCAAAGTGCACGGGGTATACCGTTCTCGTGGTCTTGCACAACATGAACCCTCGCCGAGA
  TCTGGGGGGCAACATAGCTAATCTCAAGATCCAGAGCAAGATGCACATAATATCACCCAGAATGTATCCC
  TCACAACTGAACAGATTTGTGAACACTTACACCAAAGGGCTGCCAGTGGCGATCAGCCTCCTCCTGAAAG
  ATATTGTCCAGCACCACGCATTGAGGCCCTGCTATGATTGGGTCATCTATAATACCACTCCTGAACAGGA
  AGCCTTGCAGTGGTCTTACCTCCATCCGAGGGATGGATTGATGCCCATGTATTTGAATATCCAAAGCCAT
  CTCTATCGCGTGCTTGAAAAAATTCATCGGGTGCTTAATGACAGGGATAGATGGAGTCGAGCGTATCGAG
  CCAGGAAAATTAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100213 representing AP_000538
  Red=Cloning sites Green=Tags
  
  METKGRRSAAVLDQQDESEAHPRKRPTRRAPIHRDGNNPDSDAATMEGSDPGSAGRPSSDSLLQEPSQPA
  KRGGLLDRDAIEHITELWDRLELLQQTLSKMPMADGLKPLKNFSSLQELLSLGGERLLTDLVRENIHVRE
  MMNEVAPLLREDGSCRSLNYHLQPVIGVIYGPTGCGKSQLLRNLLSSQLITPAPETVFFIAPQVDMIPPS
  ELKAWEMQICEGNYAPGPEGTFIPQSGTLRPKFIKMAYDDLTQEHNYDVSDPRNVFARAAAHGPIAIIMD
  ECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDLGGNIANLKIQSKMHIISPRMYP
  SQLNRFVNTYTKGLPVAISLLLKDIVQHHALRPCYDWVIYNTTPEQEALQWSYLHPRDGLMPMYLNIQSH
  LYRVLEKIHRVLNDRDRWSRAYRARKIK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000538
• RefSeq ORF: 1344
• MW: 50.7 kDa