E1B 55K (AC_000018) Virus Tagged ORF Clone

CAT#: VC100184

Myc-DDK-tagged ORF clone of viral ORF for E1B 55K [Human adenovirus 7], codon optimized for human cell expression, AP_000536

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B 55K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100184 represents NCBI reference of AP_000536 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCGCCTAACTCTCTCCAGCAGGGGATCCGGTTCGGGTTTCATAGCAGTTCCTTTGTTGAGAATA
  TGGAGGGATCCCAGGATGAAGATAACCTGAGACTGCTGGCATCCGCCGCAAGTAGAAGCAGCCGAGATAC
  TGAAACTCCGACCGACCACGCCTCCGGAAGCGCGGGTGGAGCAGCTGGTGGCCAGTCAGAATCCAGGCCC
  GGACCCAGTGGCGGTGGGGTAGCTGATCTCTTCCCTGAACTGCGCAGGGTGCTGACCAGGAGCACAACAA
  GTGGACAGAATCGAGGTATCAAAAGAGAGCGAAATCCCAGCGGTAATAACTCACGGACCGAACTGGCATT
  GTCTCTGATGTCCAGAAGGCGCCCTGAAACCGTTTGGTGGCACGAAGTCCAGTCTGAGGGACGGGACGAA
  GTCTCCATCCTGCAGGAAAAATACTCCCTGGAACAACTGAAGACATGTTGGCTGGAGCCCGAGGACGATT
  GGGAAGTGGCTATCAGGAATTATGCAAAGATTTCACTTAGACCTGACAAACAGTATAGAATTACAAAGAA
  AATCAATATCAGAAACGCCTGCTATATCAGCGGGAATGGAGCCGAAGTCATCATCGACACTCAGGATAAG
  GCTGCATTTCGATGCTGTATGATGGGGATGTGGCCTGGAGTCGTGGGGATGGAAGCCGTAACCTTGATGA
  ACATAAGATTTCGGGGTGATGGTTACAATGGCATCGTGTTTATGGCGAATACGAAGCTGATCTTGCATGG
  CTGCTCATTCTTTGGCTTCAATAACACCTGTGTGGAAGCATGGGGACAGGTGTCAGTGCGGGGCTGTTCC
  TTCTATGCCTGCTGGATTGCGACGTCCGGCCGCGTGAAGAGCCAACTCTCAGTGAAAAAGTGCATGTTCG
  AACGCTGCAACCTGGGCATCCTTAATGAGGGGGAAGCCAGAATCCGCCACTGTGCCGCCACAGAGACTGG
  GTGTTTTATCCTCATTAAGGGCAACGCTTCAGTTAAACATAACATGATTTGCGGCCACTCTGATGAAAGG
  CCTTATCAAATGCTCACCTGCGCGGGCGGACATTGCAACATTCTGGCAACTGTGCACATTGTGAGTCATG
  CCAGAAAAAAGTGGCCAGTGTTCGAACACAACGTGATTACAAAATGTACGATGCATATTGGGGGGAGGAG
  GGGAATGTTCATGCCATACCAGTGCAACATGAACCACGTTAAGGTGATGCTTGAGCCCGATGCGTTCTCT
  CGAGTTAGCCTGACCGGAATCTTTGATATGAATATACAGCTGTGGAAAATATTGAGGTATGACGATACGA
  AACCAAGGGTTCGGGCTTGCGAGTGTGGAGGGAAGCATGCAAGGTTCCAGCCCGTGTGCGTGGACGTGAC
  CGAGGACCTGCGCCCTGACCATCTGGTCCTGGCTTGCACCGGCGCTGAATTTGGCTCTAGCGGGGAAGAG
  ACTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100184 representing AP_000536
  Red=Cloning sites Green=Tags
  
  MDPPNSLQQGIRFGFHSSSFVENMEGSQDEDNLRLLASAASRSSRDTETPTDHASGSAGGAAGGQSESRP
  GPSGGGVADLFPELRRVLTRSTTSGQNRGIKRERNPSGNNSRTELALSLMSRRRPETVWWHEVQSEGRDE
  VSILQEKYSLEQLKTCWLEPEDDWEVAIRNYAKISLRPDKQYRITKKINIRNACYISGNGAEVIIDTQDK
  AAFRCCMMGMWPGVVGMEAVTLMNIRFRGDGYNGIVFMANTKLILHGCSFFGFNNTCVEAWGQVSVRGCS
  FYACWIATSGRVKSQLSVKKCMFERCNLGILNEGEARIRHCAATETGCFILIKGNASVKHNMICGHSDER
  PYQMLTCAGGHCNILATVHIVSHARKKWPVFEHNVITKCTMHIGGRRGMFMPYQCNMNHVKVMLEPDAFS
  RVSLTGIFDMNIQLWKILRYDDTKPRVRACECGGKHARFQPVCVDVTEDLRPDHLVLACTGAEFGSSGEE
  TD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000536
• RefSeq ORF: 1476
• MW: 54.8 kDa