E3 14.7K (AC_000018) Virus Tagged ORF Clone

CAT#: VC100206

Myc-DDK-tagged ORF clone of viral ORF for E3 147K [Human adenovirus 7], codon optimized for human cell expression, AP_000562

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 14.7K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100206 represents NCBI reference of AP_000562 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGAGATTCTGACGACCAGCAATTCAGCAGAGGACCTGCTCGACATGGACGGAAGAGTGAGCGAAC
  AGAGACTGGCTCAGCTTAGGATCAGGCAGCAGCAAGAGCGGGTCACTAAGGAACTTAGAGACGTTATTCA
  GATTCACCAGTGTAAAAAAGGCATTTTCTGCCTGGTTAAGCAAGCAAAGATCAGCTATGAAATAACAGCC
  ACTGACCATCGGTTGAGTTACGAGTTGGGTCCGCAGCGCCAGAAGTTCACATGTATGGTGGGCATAACAC
  CGATTGTGATCACCCAGCAGAGTGGCGATACCAAGGGCTGCATCCACTGTTCATGCGATTCCATTGAATG
  CACATACACACTCCTGAAAACCCTCTGCGGGCTGAGGGACCTCCTCCCCATGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100206 representing AP_000562
  Red=Cloning sites Green=Tags
  
  MTEILTTSNSAEDLLDMDGRVSEQRLAQLRIRQQQERVTKELRDVIQIHQCKKGIFCLVKQAKISYEITA
  TDHRLSYELGPQRQKFTCMVGITPIVITQQSGDTKGCIHCSCDSIECTYTLLKTLCGLRDLLPMN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 405 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000562
• RefSeq ORF: 405
• MW: 15.3 kDa