V (AC_000018) Virus Tagged ORF Clone

CAT#: VC100216

Myc-DDK-tagged ORF clone of viral ORF for V [Human adenovirus 7], codon optimized for human cell expression, AP_000545

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: V
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100216 represents NCBI reference of AP_000545 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAGCGAAAGTACAAGGAGGAAATGTTGCAGGTCATTGCCCCTGAGATCTATGGGCCACCAGTGA
  AGGACGAAAAAAAACCACGGAAGATTAAGAGGGTTAAAAACAACAAGAAAGAAGAGGACGATGACGATGG
  CCTGGTCGAGTTCGTTCGCGAATTTGCCCCCAGAAGACGAGTGCAGTGGCGAGGGCGAAAGGTTCGACAG
  GTGCTCAGACCTGGCACAACTGTCGTCTTCACTCCAGGGGAACGCTCAAGCACAACATTCAAAAGATCAT
  ACGACGAGGTGTACGGGGACGATAACATACTGGAGCAGGCAGCTGACAGGCTGGACGAATTTGCATATGG
  GAAACGGACCAGGAGTAGTCCTAAGGAAGAGGCCGTGAGTATTCCACTGGATCACGGTAATCCTACGCCA
  AGCCTGAAGCCAGTGACACTCCAGCAGGTTCTGCCCGTACCTGCACGGAGGGGTGTGAAGCGCGAAGGAG
  AAGACCTCTACCCCACTATGCAGCTCATGGTCCCTAAACGGCAGCGCTTGGAGGATGTGCTGGAGAAAAT
  GAAAGTTGACGCCGACATCCAGCCCGAGGTTAAGGTCCGACCGATTAAGGAGGTGGCTCCCGGCCTTGGT
  GTGCAAACCTTCGATATCAAAATACCTACGGAATCCATGGAAGTGCAGACTGAGCCCGCAAAACCAACCA
  CAACCTCTATAGAAGTGCAGACCGAACCATGGATGCCCGCCCCGGTTGCCGCACCGTCCACCACTAGAAG
  AAGTAGGCGCAAGTACGGACCGGCAAGTCTGCTTATGCCTAACTACGCTCTGCATCCTAGCATCATCCCT
  ACCCCCGGCTATAGAGGAACCCGCTACTACCGAAACCGCTCCAACACCAGCCGGAGGAGGAAACCACCAG
  CTAGCAGAACAAGAAGGCGACGGAGGCGGAATACAGCTTCCAAGCTCACACCTGCCGCCCTGGTCCGCCG
  GGTATACCGGGACGGGCGGGCCGAGCCTCTGACACTCCCAAGGGCACGGTACCACCCCAGCATCACGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100216 representing AP_000545
  Red=Cloning sites Green=Tags
  
  MSKRKYKEEMLQVIAPEIYGPPVKDEKKPRKIKRVKNNKKEEDDDDGLVEFVREFAPRRRVQWRGRKVRQ
  VLRPGTTVVFTPGERSSTTFKRSYDEVYGDDNILEQAADRLDEFAYGKRTRSSPKEEAVSIPLDHGNPTP
  SLKPVTLQQVLPVPARRGVKREGEDLYPTMQLMVPKRQRLEDVLEKMKVDADIQPEVKVRPIKEVAPGLG
  VQTFDIKIPTESMEVQTEPAKPTTTSIEVQTEPWMPAPVAAPSTTRRSRRKYGPASLLMPNYALHPSIIP
  TPGYRGTRYYRNRSNTSRRRKPPASRTRRRRRRNTASKLTPAALVRRVYRDGRAEPLTLPRARYHPSITT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 1050 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000545
• RefSeq ORF: 1050
• MW: 40.3 kDa