hexon (AC_000018) Virus Tagged ORF Clone

CAT#: VC100192

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus 7], codon optimized for human cell expression, AP_000548

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hexon
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100192 represents NCBI reference of AP_000548 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACTCCCTCTATGATGCCCCAATGGGCATACATGCATATCGCAGGACAGGACGCCAGCGAATATC
  TGTCACCCGGGCTCGTGCAGTTTGCTCGCGCTACCGACACCTACTTTTCAATGGGGAATAAGTTCAGGAA
  CCCCACCGTGGCACCAACTCACGATGTGACAACTGACAGATCTCAGAGATTGATGCTCCGGTTCGTGCCT
  GTTGATAGAGAGGACAATACCTATAGTTACAAGGTTAGGTATACACTGGCTGTGGGCGATAACAGGGTGC
  TGGACATGGCCAGTACCTTTTTCGATATAAGAGGTGTGCTCGATCGAGGACCAAGCTTTAAGCCCTATAG
  TGGGACTGCTTATAACAGCCTGGCGCCAAAGGGCGCTCCAAACACCAGCCAGTGGATAGTCACAACCGGG
  GAGGATAACGCTACAACCTATACATTCGGCATTGCCTCCACCAAAGGCGACAACATAACAAAGGAAGGCC
  TTGAAATCGGAAAGGACATAACCGCCGATAACAAGCCCATCTACGCAGATAAGACCTACCAGCCTGAGCC
  GCAGGTCGGGGAGGAATCCTGGACAGACATTGACGGGACGAATGAAAAGTTTGGGGGACGAGCCTTGAAG
  CCAGCCACTAAAATGAAGCCGTGTTACGGGTCCTTTGCCCGCCCTACTAACATCAAAGGGGGCCAGGCGA
  AGAACAGGAAGGTGACTCCTACGGAGGGAGATGTTGAGGCAGAGGAACCAGACATCGATATGGAATTCTT
  TGATGGTAGGGAAGCGGCCGATGCTTTCTCACCAGAGATTGTATTGTATACCGAGAATGTCAACCTGGAG
  ACACCGGACAGCCACGTAGTTTACAAACCCGGAACCAGTGACGGAAATAGTCATGCTAACCTCGGTCAGC
  AAGCGATGCCCAATAGGCCCAATTATATTGGGTTTCGGGACAACTTTGTCGGGCTCATGTACTATAACTC
  TACCGGAAATATGGGTGTACTCGCAGGACAGGCTAGTCAGCTGAACGCTGTAGTAGACCTGCAGGACCGC
  AACACGGAATTGAGCTATCAGCTCTTGTTGGACAGTCTCGGAGACCGCAGCCGATATTTTAGCATGTGGA
  ATCAAGCAGTGGACAGCTATGATCCGGACGTGAGGATTATTGAAAATCACGGTGTTGAAGATGAGCTGCC
  CAACTACTGCTTTCCGCTGGACGGAATCGGACCCGGGAACAAGTACCAAGGTATTAAACCTCGCGATACT
  GCTTGGGAGAAGGACACCAAAGTATCAACTGCAAATGAGATAGCTATTGGAAACAACCTCGCCATGGAAA
  TCAATATACAGGCCAACCTTTGGCGCTCATTTCTTTATTCCAACGTTGCCCTGTACCTGCCAGATGTCTA
  CAAGTACACACCAACTAATATCACACTGCCAGCAAATACAAACACTTACGAGTACATGAATGGAAGAGTC
  GTCTCCCCCAGTCTGGTGGACTCATACATCAACATTGGTGCACGGTGGTCCTTGGATCCGATGGACAACG
  TGAATCCTTTTAATCATCACAGGAACGCCGGTCTTAGGTATAGGTCAATGCTCCTTGGGAACGGCAGATA
  TGTGCCATTCCATATCCAGGTCCCTCAGAAATTCTTTGCAGTAAAGAACCTTCTTCTTCTCCCGGGCAGC
  TACACCTATGAATGGAACTTTCGCAAGGATGTCAATATGGTTTTGCAGTCTTCACTTGGCAATGATCTGC
  GGACCGATGGGGCAAATATCAGTTTCACTTCCATAAACCTGTACGCAACGTTTTTCCCCATGGCACACAA
  CACGGCATCTACACTTGAGGCCATGTTGAGAAACGACACCAATGACCAGTCATTCAATGACTATCTCTCT
  GCGGCGAATATGCTCTACCCAATTCCAGCGAATGCTACTAATATACCCATCTCTATCCCAAGTCGGAATT
  GGGCTGCCTTTAGGGGATGGTCTTTTACACGACTCAAAACAAAGGAGACGCCCAGCCTGGGGAGCGGCTT
  CGATCCTTATTTTGTTTATAGCGGGAGTATTCCATACCTGGACGGTACATTTTACCTGAACCACACATTC
  AAGAAAGTTTCTATTATGTTCGACTCCTCAGTGAGCTGGCCCGGCAACGATCGACTGCTGTCTCCCAATG
  AATTCGAAATCAAGCGGACAGTGGACGGGGAGGGCTATAATGTAGCTCAGTGTAATATGACCAAAGACTG
  GTTCCTGGTCCAGATGCTGGCAAATTACAATATCGGGTACCAAGGTTTTTATATTCCGGAGGGATACAAA
  GATAGGATGTACTCATTCTTTAGGAATTTTCAACCAATGTCTCGGCAGGTGGTCGACGAGGTAAACTACA
  CCGACTATAAGGCAGTCACTCTGCCCTACCAGCATAACAACAGCGGATTCGTTGGATACCTCGCTCCCAC
  GATGCGCCAGGGTGAGCCGTACCCCGCTAATTACCCCTACCCTCTTATCGGGACCACGGCAGTGAAAAGC
  GTGACACAGAAGAAGTTCCTCTGCGACCGCACCATGTGGCGGATTCCCTTCAGTTCTAACTTCATGTCAA
  TGGGTGCCTTGACTGACCTCGGCCAGAACTTGCTGTACGCTAATTCCGCGCATGCCCTGGATATGACCTT
  CGAAGTCGACCCTATGGATGAACCGACTCTGCTGTATCTGCTGTTCGAAGTGTTCGATGTAGTCCGCGTC
  CATCAACCTCACAGAGGGGTGATCGAGGCAGTCTACCTCAGAACACCATTTTCCGCTGGCAACGCAACCA
  CC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100192 representing AP_000548
  Red=Cloning sites Green=Tags
  
  MATPSMMPQWAYMHIAGQDASEYLSPGLVQFARATDTYFSMGNKFRNPTVAPTHDVTTDRSQRLMLRFVP
  VDREDNTYSYKVRYTLAVGDNRVLDMASTFFDIRGVLDRGPSFKPYSGTAYNSLAPKGAPNTSQWIVTTG
  EDNATTYTFGIASTKGDNITKEGLEIGKDITADNKPIYADKTYQPEPQVGEESWTDIDGTNEKFGGRALK
  PATKMKPCYGSFARPTNIKGGQAKNRKVTPTEGDVEAEEPDIDMEFFDGREAADAFSPEIVLYTENVNLE
  TPDSHVVYKPGTSDGNSHANLGQQAMPNRPNYIGFRDNFVGLMYYNSTGNMGVLAGQASQLNAVVDLQDR
  NTELSYQLLLDSLGDRSRYFSMWNQAVDSYDPDVRIIENHGVEDELPNYCFPLDGIGPGNKYQGIKPRDT
  AWEKDTKVSTANEIAIGNNLAMEINIQANLWRSFLYSNVALYLPDVYKYTPTNITLPANTNTYEYMNGRV
  VSPSLVDSYINIGARWSLDPMDNVNPFNHHRNAGLRYRSMLLGNGRYVPFHIQVPQKFFAVKNLLLLPGS
  YTYEWNFRKDVNMVLQSSLGNDLRTDGANISFTSINLYATFFPMAHNTASTLEAMLRNDTNDQSFNDYLS
  AANMLYPIPANATNIPISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYFVYSGSIPYLDGTFYLNHTF
  KKVSIMFDSSVSWPGNDRLLSPNEFEIKRTVDGEGYNVAQCNMTKDWFLVQMLANYNIGYQGFYIPEGYK
  DRMYSFFRNFQPMSRQVVDEVNYTDYKAVTLPYQHNNSGFVGYLAPTMRQGEPYPANYPYPLIGTTAVKS
  VTQKKFLCDRTMWRIPFSSNFMSMGALTDLGQNLLYANSAHALDMTFEVDPMDEPTLLYLLFEVFDVVRV
  HQPHRGVIEAVYLRTPFSAGNATT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 2802 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000548
• RefSeq ORF: 2802
• MW: 105.2 kDa