fiber (AC_000018) Virus Tagged ORF Clone

CAT#: VC100207

Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus 7], codon optimized for human cell expression, AP_000564

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: fiber
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100207 represents NCBI reference of AP_000564 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAAGAGGGTGCGCCTGAGCGATTCCTTTAATCCGGTTTACCCATATGAGGACGAATCAACTTCCC
  AGCATCCCTTTATTAATCCTGGCTTTATAAGTCCCAACGGATTCACGCAGTCCCCTGATGGGGTCCTCAC
  CCTGAAATGTCTTACGCCGCTCACAACGACAGGAGGGTCCCTCCAATTGAAGGTTGGCGGGGGCCTGACC
  ATTGACGACACCGATGGGTTTCTGAAGGAAAACATTTCAGCGACCACACCGCTGGTGAAAACGGGCCATA
  GTATCGGACTGTCACTTGGCCCCGGACTGGGGACCAATGAAAACAAACTCTGCGCAAAATTGGGGGAGGG
  GCTCACGTTCAACTCAAATAACATTTGCATTAACGATAATATTAATACTCTCTGGACCGGTGTCAATCCA
  ACCAGAGCCAACTGCCAGATCATGGCAAGTTCTGAAAGCAATGACTGCAAATTGATTCTGACGCTCGTTA
  AGACTGGAGCACTGGTGACAGCCTTTGTTTATGTAATCGGAGTCAGCAACGACTTTAACATGCTGACCAC
  CCACAAGAACATCAACTTCACCGCTGAGTTGTTTTTTGATAGCACGGGCAACCTTCTCACCAGTCTGTCC
  TCTTTGAAGACTCCACTGAACCACAAGAGTGGCCAGAATATGGCTACTGGCGCCCTGACCAATGCGAAAG
  GGTTCATGCCTAGTACCACAGCCTATCCCTTCAACGTCAACTCCCGAGAGAAAGAAAATTATATATATGG
  GACCTGCTATTACACAGCGTCAGACCATACCGCCTTTCCCATAGACATTAGCGTAATGCTCAACCAACGG
  GCTCTGAACAACGAGACCTCCTACTGTATTCGAGTGACGTGGTCATGGAACACCGGCGTTGCGCCAGAGG
  TACAGACATCTGCCACGACACTCGTGACGTCTCCGTTTACTTTCTATTATATTAGGGAGGATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100207 representing AP_000564
  Red=Cloning sites Green=Tags
  
  MTKRVRLSDSFNPVYPYEDESTSQHPFINPGFISPNGFTQSPDGVLTLKCLTPLTTTGGSLQLKVGGGLT
  IDDTDGFLKENISATTPLVKTGHSIGLSLGPGLGTNENKLCAKLGEGLTFNSNNICINDNINTLWTGVNP
  TRANCQIMASSESNDCKLILTLVKTGALVTAFVYVIGVSNDFNMLTTHKNINFTAELFFDSTGNLLTSLS
  SLKTPLNHKSGQNMATGALTNAKGFMPSTTAYPFNVNSREKENYIYGTCYYTASDHTAFPIDISVMLNQR
  ALNNETSYCIRVTWSWNTGVAPEVQTSATTLVTSPFTFYYIREDD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 975 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000564
• RefSeq ORF: 975
• MW: 35.3 kDa