22K (AC_000018) Virus Tagged ORF Clone

CAT#: VC100196

Myc-DDK-tagged ORF clone of viral ORF for 22K [Human adenovirus 7], codon optimized for human cell expression, AP_000553

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 22K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100196 represents NCBI reference of AP_000553 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACACAGACGGGGGTCCAAGAAGCTGAAGGTCCAGCTCACTCCCCCAGAGGATATGGAAGAAGACT
  GGGACTCCCAGGCTGAAGAGGAGATGGAGGACTGGGACAGCCAGGCTGAGGAAGTGGATAGCCTTGAGGA
  GGATTCCCTCGAAGAGGAAGATGAGGAAGCCGAAGAGGTAGAGGAGGCAACTGCCGCCAAACAGTTGTCC
  AGCGCCGCAGAGACTTCTAAAAGCCCTGATTCCAGCACCGCTACAATCTCCGCTCCAGGCAGAGGAGTAC
  AGCGCCGCCCCAACAGTCGCTGGGATGAGACAGGCCGGTTCCCCAATCCCACCACAGCTAGCAAGACAGG
  CAAGAAGGAGCGCCAGGGCTACAAATCATGGAGGGGACACAAGAATGCCATTATATCCTGTCTCCACGAG
  TGCGGGGGAAACATATCATTCACGAGACGGTACTTGCTGTTTCATCACGGCGTGAACTTTCCCAGGAATA
  TCCTGCACTATTATCGCCACCTGCATTCACCTTATTGTTCCCAGCAGGTGCCAGCCACTCCCACCGAGAA
  AGATTCTAGCGATAACGGGGACCAGAAAACTTCTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100196 representing AP_000553
  Red=Cloning sites Green=Tags
  
  MSHRRGSKKLKVQLTPPEDMEEDWDSQAEEEMEDWDSQAEEVDSLEEDSLEEEDEEAEEVEEATAAKQLS
  SAAETSKSPDSSTATISAPGRGVQRRPNSRWDETGRFPNPTTASKTGKKERQGYKSWRGHKNAIISCLHE
  CGGNISFTRRYLLFHHGVNFPRNILHYYRHLHSPYCSQQVPATPTEKDSSDNGDQKTSS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 597 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000553
• RefSeq ORF: 597
• MW: 22.5 kDa