pol (AC_000018) Virus Tagged ORF Clone

CAT#: VC100214

Myc-DDK-tagged ORF clone of viral ORF for pol [Human adenovirus 7], codon optimized for human cell expression, AP_000539

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pol
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100214 represents NCBI reference of AP_000539 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGGTACAGGCACACGGCGCTGGTAGCTTCCATCCCGAAGCCTCTGATCCGGGCTGCCAGCCCC
  CTCGGAGAAGGACTTGCGAATCCAGTCAAGGGGCAGTGCCGGAGCCAACAAGGGCACGAAGGCGACGCAC
  TACAGCATCTCATGCCGCAGGGTCTAGGGCGGCGCCTGCCGCAAGGTGTGCGCCTAGCACTCCCCTGCTC
  AATATGCAAGAGGCTACCGAGCCCCCACCTCCAAAGAGGAAGAACAAGGGAACAGTAGTGGCCCCAAAGG
  GCCATGGGACTCTCCAGGCAATAGATATCAGTACTAACGGGCCAGTCGAGATTAAGTATCATCTTAATTT
  GCCTCACGCTCTGGAGAAAATCATGCAGGTGAACCTCCTCACACTGCCAACAAATCTGACCCCACAGCGG
  CTGAGAACTCTGGACTCATCAGGACTGCGGGCTCTGGTGCTGGAGCTGCGCCCATGCCGCGCCGAAGTTT
  GGACATGTCTCCCCAGGGGCCTGGTGAGCATGACTACGATTGAGACAGAGGATGGACATGCAGACGCTGA
  TAATATTGTCGAACGCCAGGTTCAGACCCCTGGGCTCAATTTTCCACTGAAGTTCCTGGTTAAGGGAAGC
  CAGGTCCAGTTGATCCACGAAGTGCACCCGGTTAACCGCTGTGAGTACTGCGGGCGGCTCTACAAACATA
  AGCACGAATGTTCCGCCCGACGCAGAGAGTTTTACTTCCACCACATCAACTCTCATAGCTCAAATTGGTG
  GCAGGAAATACAATTCTTTCCTATCGGATCACATCCCCGCACTGAGCGACTGTTCCTCACTTACGACGTG
  GAGACATATACTTGGATGGGTAGTTTTGGCAAACAGCTCATCCCTTTCATGCTGGTGATGAAACTCAGCG
  GCGACCAGAGGCTCGTGAACATCGCCTATGATCTGGCAATGAAACTGAAGTGGGACCGCTGGAGACAGGA
  TCCCCAGACATTCTACTGCGTGACTCCTGAAAAAATGGCAGTCGGCCAGCACTTCAGGCAATACCGAGAT
  CAATTGCAGACAGCGCTGGCGGTAGACCTGTGGTCCTCATTTCTCAAGGCTAACCCCCACGTGCATGAGT
  GGGCTCTGGAACACTACGCACTGACCGATCCTACGGATCTCACTTTCGAAGAATTGAAAAAACTGCCGCA
  TGTGAGAGGGACACCCCGGTTCCTGGAACTTTATATAGTCGGGCATAACATCAACGGCTTCGATGAAATA
  GTCCTGGCCGCACAGGTTATCAATAATCGCGCAGAAGTCCCCCAGCCCTTTAGAATCACCAGGAATTTTA
  TGCCCAGAGCTGGAAAAATACTGTTTAATGACGTTACATTCGCCTTGCCGAATCCAGCCTACAAGAAAAG
  AGTAGATTTTCAACTCTGGGAGCAGGGGGCATGTGACGACATGGATTTTAAGTACCAGTTTTTGAAAGTG
  ATGGTGCGGGACACCTTTGCTCTCACACATACCAGCCTGCGGAACGCCGCACAGGCATACTCACTTCCTG
  TGGAGAAAGGATGTTGCCCTTACAAAGCTGTAAATCAGTTCTACATGCTCGGGTCTTATCGAGCAGAGAA
  GGATGGCTTCCCGCTGGAGGAGTATTGGAAAGATCGAGAAGAGTATCTTCTGAACAGAGAGCTCTGGGAG
  AAAAAGTCTCAACCTCGATACGACATCATTCAGGAAACACTGGATTATTGCGCCCTTGATGTTCTGGTTA
  CGGCTGAGCTCGTGGCAAAGCTCCAAGAATCATACGCACACTTCATCCGCGACTCTGTAGGCCTGCCACA
  CGCACACTTTAATATATTTCAAAGACCTACAATTAGCAGCAATTCTCACGCCATTTTCAGACAGATCGTG
  TATAGAGCAGAGAAGCCTAATCGAACCAACCTGGGTCCTGGCCTCCTCGCACCTTCTCACGAACTGTACG
  ACTACGTGAGGGCTAGCATTCGAGGTGGACGCTGCTATCCGACATATATCGGCATCCTGGAAGAGCCTCT
  GTACGTCTACGACATCTGTGGCATGTATGCAAGTGCGCTGACGCACCCCATGCCATGGGGAACTCCTCTC
  AACCCGTACGAGCGAGCTCTGGCAGTTAGGGAGTGGCAGATGACCCTCGACGATCCCACAACAATTAGTT
  ACTTTGATAAAGACCTCCTGCCCGGGATCTTTACTATCGACGCTGATCCCCCTGATGAGCTTATGCTTGA
  CCCTCTCCCACCATTTTGTAGTCGGAAGGGGGGCCGCTTGTGTTGGACAAATGAGCCTCTCCGCGGCGAG
  GTGGCCACTAGCGTTGACCTGATCACCTTGCACAACCGGGGCTGGAGGGTTCGGATTGTGCCTGACGAGC
  TCACCACAATCTTTCCTGAGTGGAAGTGCGTTGCTCGAGAGTACGTGCAGCTCAATATCGCTGCCAAGGA
  GCGGGCCGACAAAGAGAAAAACCAGACCATGAGAAGCATCGCCAAACTGCTTAGCAACGCCCTGTACGGC
  TCCTTTGCCACGAAACTTGACAACAAAAAAATCGTATTTAGCGATCAGATGGATGAAAGTTTGATGAAAG
  GAATCTCCGCGGGCACCGTTAACATTAAGTCCAGTTCCTTTCTCGAAACGGACAATCTCTCTGCCGAGGT
  CATGCCCGCTTTTGAGAGAGAATATCTGCCACAGCAACTTGCCCTCCTCGACTCCGATCCTGAGGACTCA
  GAAGATGAACAGCGGCCCGCACCCTTTTACACACCACCCGCCGGTACCCCAGGCCACGTTGCTTATACCT
  ACAAACCTATCACTTTCCTCGATGTTGAAGAGGGGGATATGTGTCTGCATACCGTAGAGAAGGTGGACCC
  CTTGGTGGATAACGATAGATACCCTTCTCACGTAGCTTCCTTTGTACTTGCCTGGACCAGAGCATTTGTG
  TCCGAGTGGGCCGGATTCCTGTACGAAGAGGATAGGGGCACACCGCTGGAGGATAGACCCATCAAAAGCG
  TATACGGTGACACCGATTCTTTGTTTGTCACTCAACGGGGACACGACTTGATGGAGACAAAGGGTAAGAA
  AAGAATCAAGAAGCACGGCGGGAAGCTCGTGTTCGATCCTGATGAACCGGACCTGACCTGGCTGGTGGAA
  TGTGAGACAGTGTGCGCTAGTTGTGGTGCTGATGCATACAGCCCGGAAAGTATCTTCCTCGCACCCAAGT
  TGTACGCCCTGAAGTGCATATACTGTCCAGCATGTCACAAGACTTCTAAGGGAAAACTTCGGGCAAAGGG
  CCATGCCGCCGAGGCCCTGAACTACGAACTGATGGTGAACTGCTATCTCGCGGATATGCAAGGAGCAGAT
  CGGCAGCGCTTTTCTACATCCAGAATGAGTCTGAAGCGGACACTCGCCTCCGCTCAGCCCGGTGCACACC
  CCTTTACAGTGACCGAGACAACCCTGACACGCACGCTGCGCCCCTGGAAGGACCGCACACTGGCGGCTCT
  GGACGCCCACAGGCTGATACCATACAGCAGAAGCCGACCAAATCCTCGCAATGAAGAAGTCTGCTGGATC
  GAGATGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100214 representing AP_000539
  Red=Cloning sites Green=Tags
  
  MALVQAHGAGSFHPEASDPGCQPPRRRTCESSQGAVPEPTRARRRRTTASHAAGSRAAPAARCAPSTPLL
  NMQEATEPPPPKRKNKGTVVAPKGHGTLQAIDISTNGPVEIKYHLNLPHALEKIMQVNLLTLPTNLTPQR
  LRTLDSSGLRALVLELRPCRAEVWTCLPRGLVSMTTIETEDGHADADNIVERQVQTPGLNFPLKFLVKGS
  QVQLIHEVHPVNRCEYCGRLYKHKHECSARRREFYFHHINSHSSNWWQEIQFFPIGSHPRTERLFLTYDV
  ETYTWMGSFGKQLIPFMLVMKLSGDQRLVNIAYDLAMKLKWDRWRQDPQTFYCVTPEKMAVGQHFRQYRD
  QLQTALAVDLWSSFLKANPHVHEWALEHYALTDPTDLTFEELKKLPHVRGTPRFLELYIVGHNINGFDEI
  VLAAQVINNRAEVPQPFRITRNFMPRAGKILFNDVTFALPNPAYKKRVDFQLWEQGACDDMDFKYQFLKV
  MVRDTFALTHTSLRNAAQAYSLPVEKGCCPYKAVNQFYMLGSYRAEKDGFPLEEYWKDREEYLLNRELWE
  KKSQPRYDIIQETLDYCALDVLVTAELVAKLQESYAHFIRDSVGLPHAHFNIFQRPTISSNSHAIFRQIV
  YRAEKPNRTNLGPGLLAPSHELYDYVRASIRGGRCYPTYIGILEEPLYVYDICGMYASALTHPMPWGTPL
  NPYERALAVREWQMTLDDPTTISYFDKDLLPGIFTIDADPPDELMLDPLPPFCSRKGGRLCWTNEPLRGE
  VATSVDLITLHNRGWRVRIVPDELTTIFPEWKCVAREYVQLNIAAKERADKEKNQTMRSIAKLLSNALYG
  SFATKLDNKKIVFSDQMDESLMKGISAGTVNIKSSSFLETDNLSAEVMPAFEREYLPQQLALLDSDPEDS
  EDEQRPAPFYTPPAGTPGHVAYTYKPITFLDVEEGDMCLHTVEKVDPLVDNDRYPSHVASFVLAWTRAFV
  SEWAGFLYEEDRGTPLEDRPIKSVYGDTDSLFVTQRGHDLMETKGKKRIKKHGGKLVFDPDEPDLTWLVE
  CETVCASCGADAYSPESIFLAPKLYALKCIYCPACHKTSKGKLRAKGHAAEALNYELMVNCYLADMQGAD
  RQRFSTSRMSLKRTLASAQPGAHPFTVTETTLTRTLRPWKDRTLAALDAHRLIPYSRSRPNPRNEEVCWI
  EMP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 3579 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000539
• RefSeq ORF: 3579
• MW: 136.0 kDa