protease (AC_000018) Virus Tagged ORF Clone

CAT#: VC100193

Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus 7], codon optimized for human cell expression, AP_000549

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: protease
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100193 represents NCBI reference of AP_000549 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGCGGCAGCGGAAATGGCTCATCAGAACAGGAACTGAAGGCTATCGTTCGGGATCTGGGATGCG
  GGCCTTATTTTCTGGGGACGTTCGACAAACGATTTCCCGGTTTCATGGCTCCCGATAAACTGGCTTGTGC
  AATTGTTAATACAGCCGGCAGAGAGACTGGAGGCGAGCATTGGCTTGCATTCGGATGGAATCCCCGATCT
  AACACTTGTTACCTGTTTGACCCATTTGGGTTTAGCGACGAGCGACTGAAACAGATTTATCAGTTTGAGT
  ACGAAGGCCTGCTGCGGAGAAGCGCACTGGCAACCAAAGACAGGTGTATCACCCTCGAAAAAAGCACCCA
  ATCAGTGCAAGGCCCGCGGAGCGCGGCATGTGGACTCTTTTGTTGTATGTTCTTGCACGCCTTTGTCCAT
  TGGCCTGATAGACCAATGAATGGGAATCCAACTATGAAGCTTCTGACCGGGGTGCCTAATTCTATGCTGC
  AGTCTCCACAGGTACAGCCTACTTTGCGGAGGAACCAGGAGGCTCTTTACCGATTTTTGAACACTCATTC
  TAGCTACTTTCGGTCTCACCGGGCACGAATAGAGCGGGCCACAGCTTTCGACAGGATGGACATGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100193 representing AP_000549
  Red=Cloning sites Green=Tags
  
  MSCGSGNGSSEQELKAIVRDLGCGPYFLGTFDKRFPGFMAPDKLACAIVNTAGRETGGEHWLAFGWNPRS
  NTCYLFDPFGFSDERLKQIYQFEYEGLLRRSALATKDRCITLEKSTQSVQGPRSAACGLFCCMFLHAFVH
  WPDRPMNGNPTMKLLTGVPNSMLQSPQVQPTLRRNQEALYRFLNTHSSYFRSHRARIERATAFDRMDMQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 627 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000549
• RefSeq ORF: 627
• MW: 23.7 kDa