E4 ORF2 (AC_000018) Virus Tagged ORF Clone

CAT#: VC100211

Myc-DDK-tagged ORF clone of viral ORF for E4 ORF2 [Human adenovirus 7], codon optimized for human cell expression, AP_000569

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4 ORF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100211 represents NCBI reference of AP_000569 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGAGAGGAGCGCCGTGACTTACTGCATTTGCATCCCAGAAGTCCTGAACGTTTCCCTGGAGAATT
  TCTCCTTTATCCAATTTTTGCAGGAGACCCTGCCTGACTACATCAGTTCAAATTTCGATGATATCACCGG
  AGGGTCTCAGTATGCTTATAGTAACCTCGTGTTCGCAGGCGCTAACTGGGGCGGCCTTCGACTCTACTGT
  ACTGTCGCCAGCCCCGCAATAGTGCCCGGGGGTCTCCTGGCAAAGCAGTTCGGTGACAATATGAAAGAAT
  ACCTCCAGCTTGAGTTGAGAGAGGAACTGAGGGCCAAGGGTATGTCTATCGATGACGCCATACTTAACCT
  GCTGCAGGTTACCCAGGAGCAGGACATCCTGACCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100211 representing AP_000569
  Red=Cloning sites Green=Tags
  
  MLERSAVTYCICIPEVLNVSLENFSFIQFLQETLPDYISSNFDDITGGSQYAYSNLVFAGANWGGLRLYC
  TVASPAIVPGGLLAKQFGDNMKEYLQLELREELRAKGMSIDDAILNLLQVTQEQDILTL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 387 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000569
• RefSeq ORF: 387
• MW: 14.4 kDa