52K (AC_000018) Virus Tagged ORF Clone

CAT#: VC100187

Myc-DDK-tagged ORF clone of viral ORF for 52K [Human adenovirus 7], codon optimized for human cell expression, AP_000541

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 52K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100187 represents NCBI reference of AP_000541 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCCTGTGCTGCGCCAGATGAGACCCCAGCAGCAGCCTCCATCCCAACAACAGCTGCAGCAGCAAC
  CACAAAAAGCGCTGCCAGCCCCCGTTACCACCGCAGCAGCTGCCGTATCAGGCGCCGGCCAGCCAGCTTA
  TGACCTGGAGCTGGAAGAAGGAGAAGGACTGGCCAGATTGGGAGCACCATCTCCAGAACGGCACCCGAGA
  GTCCAGCTGAAAAAGGATTCTCGCGAGGCATACGTTCCACAACAAAACCTGTTCAGGGACCGCAGCGGTG
  AGGAACCTGAAGAAATGCGCGCAAGTAGGTTCAATGCAGGCAGGGAACTCCGGCACGGATTGGATCGGCG
  ACGAGTATTGCAGGACGAAGATTTCGAAGTGGATGAGGTCACCGGGATCTCTCCCGCAAGGGCACACGTC
  GCCGCCGCGAACCTCGTGAGTGCTTACGAGCAAACAGTGAAGGAAGAAAGAAATTTCCAGAAATCATTTA
  ATAATCATGTTCGGACACTCATCGCCCGAGAGGAGGTCACACTGGGACTGATGCATCTCTGGGACTTGAT
  GGAAGCCATTACACAGAACCCTACTAGTAAACCCCTCACCGCCCAGCTGTTTCTGGTCGTCCAGCATTCC
  AGAGACAATGAGGCGTTTAGGGAAGCTTTGCTTAACATTACTGAACCGGACGGCCGCTGGCTCTACGACT
  TGATTAACATCCTTCAGAGCATTATCGTGCAGGAGAGATCTCTGGGACTGGCTGAGAAGGTAGCCGCCAT
  TAACTATTCTGTGCTCAGCCTGGGCAAATACTATGCGAGGAAGATTTACAAGACTCCTTACGTACCCATA
  GACAAAGAAGTGAAGATCGATGGGTTTTACATGAGAATGACACTGAAGGTCCTCACCCTGTCCGACGACC
  TCGGGGTTTACCGGAACGACAGGATGCATAGGGCTGTAAGCGCCTCCAGGAGGCGGGAGCTTAGCGATAG
  GGAACTGATGCATTCACTGCAGCGGGCGCTTACCGGTGCCGGTACAGACGGTGAAAATTACTTCGACATG
  GGTGCCGACTTGCAATGGCAGCCCAGCAGGCGGGCTATGGAGGCCGCAGGATGCGAACTGCCGTATATAG
  AGGAAGTCGACGAAGTGGAGGACGAAGAGGGAGAGTATCTTGAGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100187 representing AP_000541
  Red=Cloning sites Green=Tags
  
  MHPVLRQMRPQQQPPSQQQLQQQPQKALPAPVTTAAAAVSGAGQPAYDLELEEGEGLARLGAPSPERHPR
  VQLKKDSREAYVPQQNLFRDRSGEEPEEMRASRFNAGRELRHGLDRRRVLQDEDFEVDEVTGISPARAHV
  AAANLVSAYEQTVKEERNFQKSFNNHVRTLIAREEVTLGLMHLWDLMEAITQNPTSKPLTAQLFLVVQHS
  RDNEAFREALLNITEPDGRWLYDLINILQSIIVQERSLGLAEKVAAINYSVLSLGKYYARKIYKTPYVPI
  DKEVKIDGFYMRMTLKVLTLSDDLGVYRNDRMHRAVSASRRRELSDRELMHSLQRALTGAGTDGENYFDM
  GADLQWQPSRRAMEAAGCELPYIEEVDEVEDEEGEYLED
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 1167 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000541
• RefSeq ORF: 1167
• MW: 44.3 kDa