100K (AC_000018) Virus Tagged ORF Clone

CAT#: VC100195

Myc-DDK-tagged ORF clone of viral ORF for 100K [Human adenovirus 7], codon optimized for human cell expression, AP_000551

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 100K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100195 represents NCBI reference of AP_000551 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACCCAACCATCCTTGCCCACAAGCCTGCAGGCGCCTTCACATCTGGCCCCATCATCCGATGAGG
  AAGAGTCACTGACCACACCGCCGCCTAGCCCTGCGACAACCACGAGTACGCTGGACGACGAGGAGGAGGA
  GGTTGACGCCGCACAGGAGATGCAGGCTCAGGATAATGTAAAGGCAGAGGAGATAGAGGCTGATGTGGAG
  CAGGATCCCGGATACGTGACACCTGCTGAACATGAAGAAGAATTGAAGCGCTTTATCGATCGCGAAGACG
  ATGACAGGCCCGAGCACCAGGCTGACGGCGACCACCAGGAGGCTGGGATTGGCGACCAAGTGGCAGACTA
  CCTTACGGGGCTGGGGGGCGAGGACGTTCTCCTGAAGCACCTTGCCCGCCAGTCTAATATCGTCAAGGAC
  GCCCTGCTCGATTTGACGGAAGTACCTATATCCGTGGAAGAACTTTCCAGAGCTTACGAATTGAACCTCT
  TCTCCCCCCACATCCCGCCAAAGCGCCAGCCCAATGGTACTTGCGAAGCTAACCCCCGGCTGAATTTTTA
  TCCCGCATTCACCGTGCCAGAAGTCCTGGCGACCTATCACATCTTTTTTAAAAACCAGAAAATACCCGTC
  AGCTGTAGGGCCAATCGGACACGCGCCGACGCCCTCCTTAATCTCGGACCCGGTGCGAGACTGCCCGACA
  TCGCTTCCCTCGAAGAAGTCCCTAAGATCTTCGAGGGGCTCGGCTCCGATGAGACCCGGGCAGCCAATGC
  TCTGCAGCAAGGGGAAAATGGAATGGATGAGCACCACTCAGCCCTCGTCGAACTCGAAGGTGATAACGCA
  AGACTCGCGGTCCTGAAGCGGTCTATAGTCGTGACCCATTTCGCTTATCCCGCTGTGAATCTGCCACCTA
  AGGTGATGTCTGCCGTAATGGACCACCTTTTGATAAAGCGAGCAGGCCCACTGAGCGAAAACCAAAACAT
  GCAGGACCCAGATGCTTCAGACGAGGGAAAACCCGTCGTCAGCGATGAACAACTGTCTCGATGGCTCGGC
  ACTAACAGCCCCCGGGACCTGGAGGAGCGACGCAAGCTGATGATGGCCGTTGTGCTGGTGACTGTAGAAT
  TGGAATGTCTGCGCCGCTTCTTCACTGACCCTGAAACCCTGCGGAAGCTGGAGGAGAACCTTCATTACAC
  ATTTAGACACGGCTTCGTGCGCCAGGCATGCAAAATCAGCAACGTAGAACTGACCAACTTGGTCAGCTAC
  ATGGGGATCCTGCACGAGAATAGACTGGGACAGAGTGTTCTGCACACCACCCTCAAGGGAGAAGCGCGCA
  GGGATTACATTAGGGACTGTGTGTACCTCTACCTGTGCCATACCTGGCAAACCGGGATGGGAGTATGGCA
  GCAATGCCTGGAGGAACAGAATCTGAAAGAACTCGACAAGCTGCTTCAGAGAAGCCTGAAAGCATTGTGG
  ACAGGGTTCGACGAACGGACTGTGGCATCAGATCTCGCAGACATAATATTTCCGGAGAGGCTCCGGGTTA
  CTTTGAGGAATGGTCTGCCCGATTTCATGTCCCAGTCAATGCTGAACAACTTCCGATCCTTCATTCTGGA
  AAGGTCCGGAATACTGCCCGCTACCTGCTGTGCGTTGCCAAGTGACTTTGTTCCTTTGACATACCGGGAA
  TGTCCGCCGCCCCTGTGGTCACACTGTTATCTGTTTCGGCTTGCAAACTACCTGAGCTACCATTCTGACG
  TCATTGAGGATGTCAGCGGAGATGGCCTCCTTGAGTGCCATTGTAGGTGCAATCTTTGCACTCCCCACCG
  CTCATTGGCGTGCAATCCCCAACTGTTGTCCGAAACACAGATTATTGGCACCTTCGAGCTGCAGGGGCCT
  TCTTCCGAGGGAGAGGGGAGCAGTCCCGGTCAAAGTCTTAAGCTTACTCCTGGACTGTGGACATCAGCCT
  ATCTCAGAAAATTCCATCCTGAGGATTACCATCCATATGAGATACGCTTTTACGAGGATCAGTCTCACCC
  ACCAAAGGTAGAGCTCAGCGCGTGTGTGATAACTCAGGGAGCAATACTGGCTCAATTGCAGGCTATCCAG
  AAATCCCGACAGGAGTTCCTGCTCAAAAAGGGCAGCGGCGTGTACTTGGACCCTCAGACAGGAGAGGAAT
  TGAACACCAGGTTTCCCCAAGACGTGCCGTCTCCTCGAAAGCAAGAAGCCGAAGGCGCAGCGGATGCTCC
  AAGAGGGTACGGTGGGAGGCTGGGCCAGTCCGGAAGGGGTGGTGATGGCAGACTTGGACAGCCAGGGAGA
  GGCGGAGGCCAGCCGGGAGGACGACAATTTGGTGGTGGCCGCAGAGGAGGGAGAGGTGGCGGCAGAAGCA
  ATAGACGCCAGACCGTGGTGATTGGTGGCGGCGACAAGCAAGTGCCACGACAGCAGCACGGCTACCACTT
  GCGCTCAGGCTCCGGAGGACCCGCTGCTGCCCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100195 representing AP_000551
  Red=Cloning sites Green=Tags
  
  METQPSLPTSLQAPSHLAPSSDEEESLTTPPPSPATTTSTLDDEEEEVDAAQEMQAQDNVKAEEIEADVE
  QDPGYVTPAEHEEELKRFIDREDDDRPEHQADGDHQEAGIGDQVADYLTGLGGEDVLLKHLARQSNIVKD
  ALLDLTEVPISVEELSRAYELNLFSPHIPPKRQPNGTCEANPRLNFYPAFTVPEVLATYHIFFKNQKIPV
  SCRANRTRADALLNLGPGARLPDIASLEEVPKIFEGLGSDETRAANALQQGENGMDEHHSALVELEGDNA
  RLAVLKRSIVVTHFAYPAVNLPPKVMSAVMDHLLIKRAGPLSENQNMQDPDASDEGKPVVSDEQLSRWLG
  TNSPRDLEERRKLMMAVVLVTVELECLRRFFTDPETLRKLEENLHYTFRHGFVRQACKISNVELTNLVSY
  MGILHENRLGQSVLHTTLKGEARRDYIRDCVYLYLCHTWQTGMGVWQQCLEEQNLKELDKLLQRSLKALW
  TGFDERTVASDLADIIFPERLRVTLRNGLPDFMSQSMLNNFRSFILERSGILPATCCALPSDFVPLTYRE
  CPPPLWSHCYLFRLANYLSYHSDVIEDVSGDGLLECHCRCNLCTPHRSLACNPQLLSETQIIGTFELQGP
  SSEGEGSSPGQSLKLTPGLWTSAYLRKFHPEDYHPYEIRFYEDQSHPPKVELSACVITQGAILAQLQAIQ
  KSRQEFLLKKGSGVYLDPQTGEELNTRFPQDVPSPRKQEAEGAADAPRGYGGRLGQSGRGGDGRLGQPGR
  GGGQPGGRQFGGGRRGGRGGGRSNRRQTVVIGGGDKQVPRQQHGYHLRSGSGGPAAAQQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000018
• ORF Size: 2487 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000018.1, AP_000551
• RefSeq ORF: 2487
• MW: 92.0 kDa